Effect of metallothionein on lymphocyte apoptosis of the dairy cow cultured in vitro under heat stress

doi:10.11686/cyxb20140325

Abstract

Abstract: The effect of exogenous MT on lymphocyte apoptosis of the dairy cow was studied in vitro under heat stress. The splenic dairy cow lymphocytes were divided into 4 groups which had final concentrations of MT 0 (control group), 70 (group Ⅰ), 140 (group Ⅱ) and 210 μg/mL (group Ⅲ). The cells of each group were cultured in a cell incubator (37℃, 5%CO₂). After 20 h, the cells of each group were put into water bath pot (43℃) for 1 h, and then recultured in a cell incubator (37℃, 5%CO₂) for 3 h. Finally, various indexes were tested by observation of morphology and RT-PCR. 1) MT added in cell culture medium reduced the damage of dairy cow lymphocytes under heat stress, but the effect decreased with an increase in MT concentration. 2) The activities of CAT, SOD, GSH-Px in groups Ⅰ to Ⅲ were higher than those in the control group and the content of MDA were lower. It is suggested that MT could improve antioxidation of lymphocytes. 3) The relative transcript levels of Bax and p50 in group Ⅰ to Ⅲ were significantly (P<0.01) lower than those in the control group. The relative transcript level of Bcl-2 in groups Ⅰ to Ⅲ were significantly (P<0.01) higher than those in the control group. This indicated that MT could regulate the expression of genes related to lymphocyte apoptosis. In conclusion, exogenous MT decreased lymphocyte mortality rate, improved lymphocyte inoxidizability and restrained lymphocyte apoptosis . The best final concentration of MT was 70 μg/mL.

CLC Number:

S823.9⁺1

ZHAN Jin-shun,WU Li-zhuan,LI li-li,ZHANG Bin. Effect of metallothionein on lymphocyte apoptosis of the dairy cow cultured in vitro under heat stress[J]. Acta Prataculturae Sinica, 2014, 23(3): 215-223.

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