The effect of glutamine supplementation on the barrier function of ruminal epithelium during high grain diet feeding in dairy goats

doi:10.11686/cyxb20140436

Abstract

Abstract: The effect of protected glutamine supplementation on the barrier function of ruminal epithelium in dairy goats fed high grain diet was investigated using twelve dairy goats that were randomly allocated to 1 of 2 treatments: high-grain (HG) diet group (n=6) or glutamine (Gln) supplementation group (n=6,50 g/d Gln supplementation). After four weeks feeding,four goats in each group were slaughtered to characterize the changes in ruminal epithelial structure,tight junction (TJ) proteins mRNA expression,and inflammatory cytokines mRNA expression using H-E dyeing,scanning and transmission electron microscopy and real-time PCR. Compared with the control,Gln addition reduced (P<0.05) the concentration of propionate,and showed a trend to reduce the lactic acid concentration as well but it increased the butyrate concentration (P>0.05). Gln supplementation did not significantly influence the concentrations of acetate,isobutyl rate and total short-chain fatty acids. In addition,Gln addition up-regulated the gene expression of occludin mRNA expression (P<0.05) and reduced (P<0.05) the mRNA levels of TNF-α in the ruminal epithelia,and had a role in protecting the rumen epithelium. These results indicate that glutamine has a potential to maintain the rumen epithelial structural integrity and barrier function during high grain feeding.

CLC Number:

S827.4

LIU Yu-jie,LIU Jun-hua,XU Ting-ting,ZHU Wei-yun,MAO Sheng-yong. The effect of glutamine supplementation on the barrier function of ruminal epithelium during high grain diet feeding in dairy goats[J]. Acta Prataculturae Sinica, 2014, 23(4): 293-299.

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