Optimizing the culture conditions and determining the stability of antibiotic secretion by Polygonum viviparum of the endophytic bacteria Bacillus mojavensis

doi:10.11686/cyxb2014086

Abstract

Abstract: One strain of Bacillus mojavensis (ZA1) is known to have a strong antibacterial effect against the pathogen of potato gangrene (Phoma foveata). In this study, P. foveata was isolated as a fungal pathogen and the method of petri dish confrontation was used to determine culture conditions for optimizing and stabilizing production of the antibiotic secreted by ZA1. The results showed that the optimum culture medium for ZA1 consisted of 200 g potato, 10 g peptone, 20 g sucrose and 1000 mL distilled water. The optimum fermentation temperature of ZA1 was 17.8℃.The optimum pH value of ZA1’s culture medium was 6.9. The optimum 150 mL triangle bottle volume of ZA1 was 20 mL. The optimum culture mode of ZA1 was shaking cultivation in the dark for 96 hours. Results showed that the EC₅₀=0.1228 μL/mL against P. foveata after optimization was 37 times higher than the EC₅₀=4.5888 μL/mL against P. foveata before optimization. Crude extracting of bacteriostatic from ZA1 showed that the characteristics of high temperature resistance and relative activity could reach 76.62% after it was treated at 90℃ for 2 hours. Relative activity was stable and could not be destroyed under UV irradiation for 30 minutes. The bacteriostatic extract of ZA1 had good acid and alkali resistance. When it was treated by pH=3 and pH=11, the relative activity was 92.87% and 85.11% respectively. It was not sensitive to protease and heavy metal ions such as Ag⁺, Cu²⁺, Zn²⁺ and Fe³⁺. Relative activity remained at 86.93% after Ag⁺ treatment.

YANG Cheng-De, CHANG Tao, XUE Li, FENG Zhong-Hong, YAO Yu-Ling, LI Ting, CHEN Xiu-Rong. Optimizing the culture conditions and determining the stability of antibiotic secretion by Polygonum viviparum of the endophytic bacteria Bacillus mojavensis[J]. Acta Prataculturae Sinica, 2015, 24(9): 104-112.

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