Isolation of cellulose-degrading bacteria and determination of their degradation activity

doi:10.11686/cyxb2014172

Acta Prataculturae Sinica ›› 2016, Vol. 25 ›› Issue (3): 60-66.DOI: 10.11686/cyxb2014172

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Isolation of cellulose-degrading bacteria and determination of their degradation activity

JIA Hui¹, CHEN Xiu-Rong^{1, *}, LU Guang-Xin^{2, *}, KONG Ya-Li¹, YANG Cheng-De¹

1.Key Lab of Grassland Ecosystem of MOE, College of Prataculture, Gansu Agricultural University, Lanzhou 730070, China;
2.Department of Grassland Science,Agriculture and Animal Husbandry College of Qinghai University, Xining 810016, China

Received:2014-04-08 Online:2016-03-20 Published:2016-03-20

Abstract

Abstract: Eight bacterial strains were isolated from soil in the east Qilian Mountains. Of these, four strains had a higher capacity for degradation of plant cellulose when cultured on a CMC-Na plate medium. The growth characteristics of the four strains were studied, and the optimum temperature range of the 4 active strains was 25-30℃, the optimum pH for growth was 5-8, and the optimum sodium concentration was 4% to 5%. A strain designated X1-2 possessed the highest cellulose degradation ability. Based on morphology, gram stain reaction, and 16S rRNA phylogenetic comparison, this strain was identified as a member of the genus Bacillus (Bacillus sp.). It is a bacterium with strong plant cellulose degradation ability.

JIA Hui, CHEN Xiu-Rong, LU Guang-Xin, KONG Ya-Li, YANG Cheng-De. Isolation of cellulose-degrading bacteria and determination of their degradation activity[J]. Acta Prataculturae Sinica, 2016, 25(3): 60-66.

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