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Acta Prataculturae Sinica ›› 2015, Vol. 24 ›› Issue (6): 159-167.DOI: 10.11686/cyxb2014274

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Effects of pod pre-treatments on the germination traits of Hedysarum polybotrys seeds

SUN Yun-Bo1, 4, CHEN Xiang2, CHEN Yuan1, *, ZHOU Chuan-Meng1, BAI De-Tao3   

  1. 1.Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Gansu Provincial Key Laboratory of Good Agricultural Productive Technology for Traditional Chinese Medicines, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;
    2. Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China;
    3.Gansu Engineering Laboratory of Resource Reservation and Utilization for Characteristic Medical Plants, Gansu Cultivated Engineering and Technology Research Center of Standardization and Traceability for Characteristic Chinese Medicine, Longxi Zhongtian Pharmaceutical Co., Ltd, Dingxi 748100, China;
    4.The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China
  • Received:2014-06-09 Online:2015-06-20 Published:2015-06-20

Abstract: Plant pod seeds of Hedysarum polybotrys were used in a germination test in order to explore the effect of seed pre-treatment methods. Several methods, such as soaking the peeled seeds, and soaking and stewing the unpeeled seed pods for 12 h, were compared with non-soaked, dry seed pods as a control. Results under natural illumination at 18-20℃ showed that germination dynamics in all pre-treatments followed S curve patterns that fitted to a logistic equation. Germination started days 2-6 and finished days 14-19. The pre-treatments showed significant differences in germination traits. The peeled and soaking pre-treatment and the soaking and stewed unpeeled-seed pods pre-treatment delayed peak germination by two days. The peeled and soaking pre-treatment significantly expedited germination while the unpeeled and stewing pre-treatment prolonged germination duration. All the pre-treatments for 12 h significantly improved germination quality compared with the control. The control had a germination rate of 34.7%, germination power 4.0% and germination index 1.865. The results for the peeled and soaked seeds were 80.7%, 54.0% and 7.083 respectively, that for the unpeeled stewed seed pods 64.0%, 13.3% and 3.462 respectively. The results thus show that pre-treating seeds by soaking or stewing can significantly improve germination quality. Improvements were highest in the peeled pre-treatment, where seeds showed over 80% germination rate.