Allelopathic effect of extracts from the fibrous roots of Coptis chinensis on Lactuca compositae, Vigna radiate and Brassica rapa pekinensis

doi:10.11686/cyxb2014287

Abstract

Abstract: A study has been undertaken of the allelopathic effect of extracts from fibrous roots of Coptis chinensis (ERC) on seed germination and seedling growth in Lactuca compositae, Vigna radiate and Brassica rapa pekinensis. Seeds of the three species were cultured in plates with various concentrations of ERC and germination rate, young seedling growth and related physiological indexes were measured. There was no significant effect of ERC on seed germination rate and index at low concentrations (200 mg/L), but seed vitality decreased and seedling biomass and root vitality dropped greatly. As the concentration of ERC increased to>400 mg/L, seed starch and protein hydrolyses were inhibited and the contents of free amino acids, soluble sugars and soluble phosphorus decreased, resulting in poor germination. When ERC concentration reached 800 mg/L, roots deformed concomitantly with brown color, root hair disappearance and the curling-up of root tips to leave the ERC solution. Nitrate reductase activity and chlorophyll concentration also decreased in the leaves. As a result, seedling growth was stunted. Stronger inhibition of ERC on L. compositae was observed than on V. radiate and B. rapa pekinensis. The results thus show that allelopathic chemicals contained in root exudate and residual of C. chinensis could inhibit seed germination and seedling growth in these three plants. This inhibition could result in poor growth and yield reduction of consequent crops after intensive cultivation of C. chinensis.

WANG Ya-Qi, CHEN Dan-Mei, YUAN Ling. Allelopathic effect of extracts from the fibrous roots of Coptis chinensis on Lactuca compositae, Vigna radiate and Brassica rapa pekinensis[J]. Acta Prataculturae Sinica, 2015, 24(6): 142-149.

References

[1] Dong Y, Zhang Y F, Yang Q, et al . Study on the absorption of Coptis chinensis extract in everted gut experiment in rats. China Journal of Chinese Materia Medica, 2008, 33(9): 1056.
[2] Zhao M, Gao L, Peng X M, et al . Effect of Coptis chinensis extract on proliferation of keratinocytes. Chinese Information of Traditional Chinese Medicine Journal, 2010, 17(8): 22.
[3] Li X G, Yang L G, Chen L X, et al . Huang Lianshui, isolation and identification of chemical constituents of liquid. Journal of Shenyang Pharmaceutical University, 2012, 29(3): 193-226.
[4] Sun J, Ma J S, Jin J, et al . Qualitative and quantitative deter-mination of the main components of huanglianjiedu decoction by HPLC-UV/MS. Acta Pharmaceutical Sinica, 2006, 41(4): 380.
[5] Kuang Y H, Zhu J J, Wang Z M, et al . A determination of berberine in Coptis chinensis Franch, multi evaluation method ba Martin, coptisine, berberine, jatrorrhizine content meter. Chinese Pharmaceutical Journal, 2009, 44(5): 390-394.
[6] Yin F J, Qu X Y, Zeng W, et al . Study on auto toxicity of aquatic extracts from different parts of Coptis chinensis . Chinese Herbal Medicine, 2009, 33(3): 329.
[7] Peng S L, Wen J, Guo Q F. Progress in the research on the changes and mechanism of active substances of plant allelopathy. Acta Botanica Sinica, 2004, 46(7): 68-71.
[8] Xu J T, Wang L Q, Xu B. Research development of Coptis chinensis . Journal of China Academy of Medical Sciences, 2004, 26(6): 704.
[9] Wang X K. The Principle and Technology of Plant Physiology and Biochemistry Experiment[M]. Beijing: Higher Education Press, 2005.
[10] Yang J H, Wang C L, Dai H L. Soil Agrochemical Analysis and Environmental Monitoring[M]. Beijing: China Press, 2008.
[11] Zhang Y J, Tang S M, Shao X Q, et al . Advances of research on grassland ecosystem in plant allelopathy. Anhui Agricultural Sciences, 2012, 40(2): 958-961.
[12] Xiao Q, Zheng H L, Chen Y. Effects of salinity on the growth of Spartina alterniflora and proline, soluble sugar and protein content. Journal of Ecology, 2005, 24(4): 373-376.
[13] Fang Z H, Dong K H. Effects of NaCl stress on Artemisia anethifolia soluble sugar and soluble protein content. Chinese Agricultural Science Bulletin, 2010, 26(16): 147-149.
[14] Sudério, Fabrício B, Gomes F, et al . β-galactosidases from cowpea stems: properties and gene expression under conditions of salt stress. Revista Ciência Agronômica, 2014, 45(4): 794-804.
[15] Pan R Z. Plant Physiology[M]. Beijing: Higher Education Press, 2008.
[16] Meng Y X, Wang S H, Wang J C, et al . Effects of CoCl 2 stress on barley seedling growth and physiological index of NaCl. Acta Prataculturae Sinica, 2014, 23(3): 160-166.
[17] Yan Z, Qing Y, Juan L, et al . Growth response and nutrient uptake of Eriobotrya japonica plants inoculated with three isolates of arbuscular mycorrhizal fungi under water stress condition. Journal of Plant Nutrition, 2014, 37(5): 690-730.
[18] Li B, Zhu W L, Chen K X. Research progress of berberine hydrochloride and its derivatives. Pharmaceutical Journal, 2008, 43(8): 773-787.
[19] Peng H Y, Lin Q. Progress in the structural modification and pharmacological activity of berberine on. Strait Pharmaceutical Journal, 2010, 22(8): 7-10.
[20] Martinoia E, Massonneau A, Frangne N. Transport processes of solutes across the vacuolar membrane of higher plants. Plant Cell Physiology, 2000, 41(11): 1175.
[21] Otani M, Shitan N, Sakai K, et al . Characterization of vacuolar transport of the endogenous alkaloid berberine in Coptis chinensis . Plant Physiology, 2005, 138(4): 1939.
[22] Li H Y. The Main Effect of Pepper Allelochemicals and Allelopathy Research[D]. Beijing: Graduate University of Chinese Academy of Sciences, 2009.
[23] Xu P, Chen Z H, Liang J. Effect of different components of root exudates allelopathy of lily. Journal of Northwest Forestry University of Technology (Natural Science Edition), 2011, 39(11): 167-172.
[24] Wu W, Zhao J. Research progress on nitrogen absorption by plants. Chinese Agronomy Pass, 2010, 26(13): 75-78.
[25] Xu G W, Li S, Zhao Y F, et al . Straw and nitrogen on rice root exudates and study the influence of nitrogen utilization. Acta Prataculturae Sinica, 2014, 23(2): 140-146.
[26] Zhang J Z, Zhang Q Y, Sun G F, et al . Effects of drought stress and rewatering on the growth and photosynthesis of plants. Acta Prataculturae Sinica, 2014, 23(1): 167-176.
[1] 董宇, 张英丰, 杨庆, 等. 黄连提取物在大鼠肠外翻实验中的吸收研究. 中国中药杂志, 2008, 33(9): 1056.
[2] 赵茉, 高莉, 彭晓明, 等. 黄连提取物对角质形成细胞增殖活性的影响. 中国中医药信息杂志, 2010, 17(8): 22.
[3] 李雪改, 杨立国, 陈丽霞, 等. 黄连水提液化学成分的分离与鉴定. 沈阳药科大学学报, 2012, 29(3): 193-226.
[5] 匡艳辉, 朱晶晶, 王智民, 等. 一测多评法测定黄连中小檗碱、巴马汀、黄连碱、表小檗碱、药根碱含量. 中国药学杂志, 2009, 44(5): 390-394.
[6] 银福军, 瞿显友, 曾纬, 等. 黄连不同部位水浸液自毒作用研究. 中药材, 2009, 33(3): 329.
[7] 彭少麟, 文军, 郭勤峰. 植物化感物质活性变化及其作用机理研究进展. 植物学报, 2004, 46(7): 68-71.
[8] 徐锦堂, 王立群, 徐蓓. 黄连研究进展. 中国医学科学院学报, 2004, 26(6): 704.
[9] 王学奎. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2005.
[10] 杨剑虹, 王成林, 代亨林. 土壤农化分析与环境监测[M]. 北京: 中国大地出版社, 2008: 3.
[11] 张玉娟, 唐士明, 邵新庆, 等. 植物化感作用在草地生态系统中的研究进展. 安徽农业科学, 2012, 40(2): 958-961.
[12] 肖强, 郑海雷, 陈瑶. 盐度对互花米草生长及脯氨酸、可溶性糖和蛋白质含量的影响. 生态学杂志, 2005, 24(4): 373-376.
[13] 方志红, 董宽虎. NaCl胁迫对碱蒿可溶性糖和可溶性蛋白含量的影响. 中国农学通报, 2010, 26(16): 147-149.
[15] 潘瑞炽. 植物生理学[M]. 北京: 高等教育出版社, 2008.
[16] 孟亚雄, 王世红, 汪军成, 等. CoCl 2 对NaCl 胁迫下大麦生长及幼苗生理指标的影响. 草业学报, 2014, 23(3): 160-166.
[18] 李波, 朱维良, 陈凯先. 小檗碱及其衍生物的研究进展. 药学学报, 2008, 43(8): 773-787.
[19] 彭华毅, 林菁. 小檗碱的结构修饰及其药理学活性研究进展. 海峡药学, 2010, 22(8): 7-10.
[22] 李洪益. 花椒主效化感物质分离鉴定及化感作用研究[D]. 北京: 中国科学院研究生院, 2009.
[23] 徐鹏, 程智慧, 梁静. 百合根系分泌物中不同组分的化感作用. 西北林科技大学学报(自然科学版), 2011, 39(11): 167-172.
[24] 吴巍, 赵军. 植物对氮素吸收利用的研究进展. 中国农学通报, 2010, 26(13): 75-78.
[25] 徐国伟, 李帅, 赵永芳, 等. 秸秆还田与施氮对水稻根系分泌物及氮素利用的影响研究. 草业学报, 2014, 23(2): 140-146.
[26] 张金政, 张起源, 孙国峰, 等. 干旱胁迫及复水对玉簪生长和光合作用的影响. 草业学报, 2014, 23(1): 167-176.