Abstract: The aim of this experiment was to study the effect of dietary crude protein level on growth performance, meat quality and serum parameters of Huai pigs. Forty-eight Huai pigs (half males and half females) with similar body weight were randomly divided into 3 groups, and fed with low (11.96%), medium (13.04%) or high protein (14.16%) diets. Each treatment group had 4 replicates with 4 pigs in each replicate. The trial lasted for 138 days. The key results were: 1) No significant difference was seen among the three groups in final body weight, average daily feed intake, average daily gain and slaughter traits. However, the ratio of feed to gain in the high protein group was significantly higher than for the remaining two groups (P<0.05); 2) The pH value, color score, driage, DM, crude protein, and ash in muscle and intra-muscular pressure showed no significant difference among the three groups, whereas the content of crude fat and cholesterol in muscle were significantly increased in the high protein group (P<0.05); 3) There was no significant difference between the three groups in fatty acid, linolenic acid or arachidonic acid levels. The content of linolenic acid in the muscle of the high protein group was significantly higher than that in the low protein group (P<0.05), while arachidonic acid content of muscle was significantly lower in the high protein group than in the remaining groups (P<0.05); 4) The serum albumin levels of animals in the low protein group were significantly higher than those of medium protein group, and blood urea nitrogen in serum tended to increase with increased protein level in the diet (P<0.1). In conclusion, the medium protein diet with 13.04% crude protein was optimal for growth performance, meat quality and serum parameters during the final stage of pig fattening.