Isolation and identification of low temperature-tolerant lactic acid bacteria from legume silages in Tibet

doi:10.11686/cyxb2014312

Abstract

Abstract: The objectives of this study were to identify lactic acid bacteria with desirable attributes by isolating and identifying lactic acid bacteria (LAB) from legume silages in Tibetan Plateau. A total of 6 homofermentiative lactic acid bacteria were isolated from alfalfa (Medicago sativa) and common vetch (Vicia sativa) silage. Strains LCG9, CG35 and AG11 were identified as Pediococcus pentosaceus, strains LCG3 and LAG1 as Lactobacillus plantarum and strain LA3 as Lactobacillus curvatu. Conventional microbial identification methods and 16S rRNA sequencing analyses were used to identify different strains. The selected strains were grown in a range of conditions; temperature 5-20℃, pH 3.0-8.0 and NaCl 3.0% and 6.5%. LCG9 and AG11 grew weakly at 5℃ while LCG9 and AG11 were inactive at pH 3.0 and pH 8.0. LCG3 was identified as the best strain to use as a silage additive in Tibet due to its unique tolerance of low temperature, high lactic acid production and rapid reproductive rate.

YANG Xiao-Dan, YUAN Xian-Jun, GUO Gang, CUI Zhao-Ming, LI Jun-Feng, BAI Xi, BA Sang, SHAO Tao. Isolation and identification of low temperature-tolerant lactic acid bacteria from legume silages in Tibet[J]. Acta Prataculturae Sinica, 2015, 24(6): 99-107.

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