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Acta Prataculturae Sinica ›› 2015, Vol. 24 ›› Issue (9): 154-162.DOI: 10.11686/cyxb2014428

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Change in starch grains and flower bud differentiation of Lilium pumilum bulbs during breaking of dormancy under refrigerated conditions

LIU Fang1, 2, TIAN Zhong-Ping1, CAI Ying-Jie1, ZHANG Yu1, ZHOU Yun-Wei1, *   

  1. 1.College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China;
    2.College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Received:2014-10-20 Online:2015-09-20 Published:2015-09-20

Abstract: Changes in starch grains and flower bud differentiation of lily (Lilium pumilum) were studied during the breaking of dormancy at 5℃. Observations of paraffin sections under a microscope revealed that the height of the growth cone and the width of apical buds increased gradually during cold storage. The flower bud differentiation process in lily could be divided into four stages: the floral primordium differentiation stage from 0 to 48 days, the outer perianth primordium differentiation stage at 60 days, the inner perianth primordium differentiation stage at 72 days, and the stamen and pistil primordium differentiation stage at 84 days. The number of starch grains in apical bud cells gradually decreased as the duration of the cold temperature treatment extended. There were almost no mitotic cells in the plant tissues from 0 to 24 days of cold storage. The number of mitotic cells increased gradually after 36 days in cold storage, reaching 2.6 at 84 days of cold storage.