Optimization of fermentation conditions of swine manure and screening for efficient microbial deodorant strains

doi:10.11686/cyxb2014513

Abstract

Abstract: By combining NH₃ and H₂S selective media for a first qualitative screening, and the boric acid absorption and zinc amine complex salt absorption colorimetric methods for a second quantitative screening, 5 strains which could efficiently reduce NH₃ release, and 3 strains reducing H₂S release were identified, of which a strain designated BX3 achieved emission reduction rates of NH₃ and H₂S, respectively, compared with control treatments, of 80.07%, 76.92%. An L₈(2⁷) orthogonal optimum design was employed to identify combinations of different strains with high deodorizing efficiency, and AF2+DZ1+BX3+DZ3+BZ1+EZ3+AX4 was found to be the optimal strain combination. With this combination, the emission rates of NH₃ and H₂S were significantly lower than that of other combinations. Compared with a blank control group, the emission reductions of NH₃ and H₂S, respectively, were 82.14%, 80.84% on the fifth day of the experiment. The optimal microbial combination for deodorizing activity was studied under differing fermentation conditions and the quantities of NH₃ and H₂S released were smallest with additive combinations of 10% inoculation, 30% moisture content and 10% bran addition, and 15% inoculation, 40% moisture content and 10% bran addition, respectively. The microbial strains isolated in this study have potentially broad application prospects in the fields of odor prevention and control of environmental pollution caused by livestock and poultry manure.

ZHANG Sheng-Wei, YAO Tuo, HUANG Wang-Zhou, YANG Qiao-Li, GUN Shuang-Bao. Optimization of fermentation conditions of swine manure and screening for efficient microbial deodorant strains[J]. Acta Prataculturae Sinica, 2015, 24(11): 38-47.

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