Abstract: In this study, a 6-trehalose phosphate synthase gene (TPS) fragment, 1153 bp in length, was isolated through NaCl and NaHCO₃ screening from Puccinellia tenuiflora yeast cDNA library. A 3'-end cDNA amplification technique was used to clone PutTPS. The full-length cDNA is 3358 bp containing an open reading frame (ORF) of 882 amino acids. Multiple alignment analysis based on amino acids of TPS proteins of rice, Arabidopsis, maize, and other higher plants revealed that the amino acid homologies were about 60% to 90%.The tissue expression patterns of P. tenuiflora PutTPS and the expression under NaCl and NaHCO₃ stress were further investigated by northern blot. Also the recombinant yeast INVScI (pYES₂-PutTPS) and control INVScI (pYES₂) were subjected to salt, drought, osmotic and oxidative stresses. PutTPS was highly expressed in roots and flowers; in roots and leaves, NaCl and NaHCO₃ induced up-regulated expression of PutTPS genes and the recombinant yeast cells showed higher stress resistance than control cells. It was also found that the TPS from alkali grass contributes to salt, drought, osmotic and oxidative resistance.The present study indicated that there was a relationship between PutTPS expression and multiple stresses, and PutTPS may play an important role for P. tenuiflora during adaption to environmental abiotic stress.