Relationship between dormancy breaking and protective enzymes in refrigerated Lilium pumilum bulbs

doi:10.11686/cyxb2015031

Abstract

Abstract: Changes in bulb protective enzyme activity and carbohydrate content of Lilium pumilum bulbs held at 5℃to break dormancy were investigated. Results showed that superoxide dismutase (SOD) activity of scales, apical buds and basal plates decreased within 0-24 days (d). Except for apical buds, peroxidase (POD) activity within 0-24 d declined in all bulb parts and catalase (CAT) activity of the outer scales, apical buds and basal plates decreased within 0-12 d. During middle and later periods, SOD, CAT and ascorbate peroxidase (ASP) increased, POD activity decreased, POD and polyphenol oxidase (PPO) activity of apical buds and basal plates also increased in the middle of the cold storage period. The roles of various metabolism related enzymes in different organs were not the same. With low temperature treatment for 36-60 d, ASP and CAT activity initially rapidly increased but later stabilized. The minimum value of SOD activity was found after 36-60 d cold storage. Starch content was negatively correlated with CAT, PPO, ASP, SOD and POD in all bulb parts; phenylalanine ammonia lyase (PAL) was positively correlated with starch in the scales. Bulb dormancy breaking started at 36 d, and was mostly complete at 60 d.

LIU Fang, LI Dan-Dan, LIAN Hua, WU Xia, CHEN Ye-Wen, LI Yu-Qi, XIE Shou-Feng, CHEN Xue-Wu. Relationship between dormancy breaking and protective enzymes in refrigerated Lilium pumilum bulbs[J]. Acta Prataculturae Sinica, 2015, 24(12): 180-187.

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