Detection of pathogenic organisms in Medicago sativa in Zhangye, Gansu Province

doi:10.11686/cyxb20150414

Abstract

Abstract: Two Medicago sativa plants with severe leaf mosaic symptoms were collected from alfalfa fields in Linze County, Gansu Province, in July 2011. The two samples were tested by enzyme-linked-immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). The tested specimens were negative for Cucumber mosaic virus, Tobacco streak virus, Watermelon mosaic virus, Potato virus X, Potato virus Y, Tobacco ringspot virus, Tomato ringspot virus and Bean yellow mosaic virus. However, Tomato mosaic virus (ToMV) and Alfalfa mosaic virus (AMV) were detected. The 687 and 351 bp fragments obtained from PCR with appropriate species-specific ToMV and AMV primers showed 99% nucleotide similarity with published ToMV CP and AMV CP gene sequences. A 805 bp PCR products was obtained using genus-specific Tobamovirus primers and the presence of ToMV was confirmed by RT-PCR-RFLP. To the best of our knowledge, this is the first report of ToMV in alfalfa.

WEN Zhao-Hui, NAN Zhi-Biao. Detection of pathogenic organisms in Medicago sativa in Zhangye, Gansu Province[J]. Acta Prataculturae Sinica, 2015, 24(4): 121-126.

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