Quality and lactic acid bacteria of mixed corn stalk and cabbage waste silage

doi:10.11686/cyxb2015095

Abstract

Abstract: Silage made with a mixture of corn stalk (CS) and cabbage waste (CW) were mixed and ensilaged at different ratios, 29∶19 (ME₁), 27∶21 (ME₂), 25∶23 (ME₃), 23∶25 (ME₄), 21∶27 (ME₅) and 19∶29 (ME₆), respectively. The chemical composition and fermentation traits were measured after 30 days (d) to identify the optimal ratio of corn stalk and cabbage waste and the diversity of lactic acid bacteria in each mixture. The pH and the ratio of ammonia nitrogen to total nitrogen (AN/TN) of the ME₅ silage was significantly lower than the other mixes (P<0.05). The lactic acid content (LA) of ME₅ was significantly higher than the other mixes (P<0.05) whereas the ammonia nitrogen/total nitrogen ratio (AN/TN) of ME₅ was significantly lower than other mixes. Analysis of lactic acid bacteria showed that a total of 10 lactic acid bacteria strains were isolated from the ME₅ group, belonging to three different genera; Lactobacillus, Enterococcus and Leuconostoc. Two strains of Lactobacillus brevis, one strain of Enterococcus faecium, five strains of Leuconostoc mesenteroides subsp. mesenteroides and two strains of Lactobacillus plantarum were identified. Among these isolates, Lactobacillus and Enterococcus were homofermentative lactic bacteria, which are able to produce more lactic acid than heterofermentative lactic bacteria. Lactobacillus and Enterococcus played a key role in the fermentation of these silages. In conclusion, mixed silages of CS and CW were found to be feasible with an optimum ratio of 21∶27 respectively.

REN Hai-Wei, ZHAO Tuo, LI Jin-Ping, LI Xue-Yan, XU Na, WANG Yong-Gang, WANG Xiao-Li, GAO Xiao-Hang. Quality and lactic acid bacteria of mixed corn stalk and cabbage waste silage[J]. Acta Prataculturae Sinica, 2016, 25(1): 197-206.

References

[1] Weiland P. Biogas production: current state and perspectives. Applied Microbiology Biotechnology, 2010, 85(4): 849-860.
[2] Weinberg Z G, Chen Y. Effects of storage period on the composition of whole crop wheat and corn silages. Animal Feed Science and Technology, 2013, 185(3-4): 196-200.
[3] Zheng Y, Yu C W, Cheng Y S, et al . Effects of ensilage on storage and enzymatic degradability of sugar beet pulp. Bioresource Technology, 2011, 102(2): 1489-1495.
[4] Stevenson D M, Muck R E, Shinners K J, et al . Use of real time PCR to determine population profiles of individual species of lactic acid bacteria in alfalfa silage and stored corn stover. Applied Microbiology Biotechnology, 2006, 71(3):329-338.
[5] Yang D L, Wang J X, Feng W H, et al . Effects of broccoli stems and leaves and maize straw mix-ensiling on silage quality. Pratacultural Science, 2014, 31(3): 551-557.
[6] Li S C, Huang X H, Wang J, et al . Effects of different mixed ratio and fermenting period on efficiency of mixed silage of melilotus albus and corn straw. Pratacultural Science, 2014, 31(2): 321-327.
[7] Huang X H, Li S C, Li D H, et al . Fermentation quality and content of poisonous substances in Sophora alopecuroides and corn straw mixed silage. Pratacultural Science, 2013, 30(10): 1633-1639.
[8] Zhang F Q, Bao H F, Cui W D, et al . Dynamic changes of lactic acid bacteria during a 15-day ensilage of corn. Microbiology China, 2010, 37(6): 834-838.
[9] Tohno M, Kobayashi H, Nomura M, et al . Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage. Journal of Animal Science, 2012, 83(2): 111-120.
[10] Ennahar S, Cai Y, Fujita Y. Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis. Applied Environmental Microbiology, 2003, 69(1): 444-451.
[11] Si B W, Wang Z L, Sun Q Z, et al . Fermentation characteristics and changes of lactic acid bacteria isolated from ensiling Lespedeza hedysaroides . Pratacultural Science, 2012, 29(4): 650-657.
[12] Cai Y M, Pang H L, Kitahara M, et al . Lactobacillus nasuensissp . nov., a lactic acid bacterium isolated from silage, and emended description of the genus Lactobacillus . International Journal of Systematic and Evolutionary Microbiology, 2012, 62(5): 1140-1144.
[13] Yang Y, Shi C, Guo X S. Characterization and identification of Weissella species isolated from Kobresia littledalei growing in alpine meadows. Acta Prataculturae Sinica, 2014, 23(1): 266-275.
[14] Si B W, Wang Z L, Sun Q Z, et al . Isolation and identification of high-quality lactic acid bacteria in Hedysarum fruticosum pall. silage. Acta Agrestia Sinica, 2012, 20(1): 166-170.
[15] Weatherburn M. Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry, 1967, 39(8): 971-974.
[16] Owens V N, Albrecht K A, Muck R E, et al . Protein degradation and fermentation characteristics of red clover and alfalfa silage harvested with varying levels of total nonstructural carbohydrates. Crop Science, 1999, 39(6): 1873-1880.
[17] Shao T, Zhang Z X, Shimojo M, et al . Comparison of fermentation characteristics of Italian ryegrass ( Lolium multiflorum Lam.) and guineagrass ( Panicum maximum Jacq.) during the early stage of ensiling. Asian-Australasian Journal of Animal Science, 2005, 18(22): 1727-1734.
[18] Guo X H, Ling D W. Modern Experiment Technology of Lactic Acid Bacteria[M]. Beijing: Science Press, 2013: 285-286.
[19] Mora D, Fortina M G, Nicastro G, et al . Genotypic characterisation of thermophilic bacilli: a study on new soil isolates and several reference strains. Research Microbiology, 1998, 149(10): 711-722.
[20] Jensen M A, Webster J A, Strauss N. Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Applied Polymerase Environmental Microbiology, 1993, 59(4): 945-952.
[21] Xu C C. Silage Science and Technology[M]. Beijing: Science Press, 2013.
[22] Wang X J, Yang Y, Zhang X Q, et al . To make biofuel: cutting the lignin or loosening lignin’s grip. Scientia Agricultura Sinica, 2015, 48(2): 229-240.
[23] Liu J J, Gao L J, Shi J F, et al . Lactic acid bacteria community and Lactobacillus plantarum improving silaging effect of switch grass. Transactions of the Chinese Society of Agricultural Engineering, 2015, 31(9): 295-302.
[24] Kondo M, Kita K, Yokota H. Feeding value to goats of whole-crop oat ensiled with tea waste. Animal Feed Science and Technology, 2004, 113(1-4): 71-81.
[25] Ge J, Yang C J, Yang Z M, et al . Quality of mixed naked oats ( Avena nuda ) and alfalfa ( Medicago sativa ) silage. Acta Prataculturae Sinica, 2015, 24(4): 104-113.
[26] Jiang H, Fang L, Zhou X L, et al . Improve alfalfa silage quality by adding alhagi sparsifolia shap in initial bloom stage. Transactions of the Chinese Society of Agricultural Engineering, 2014, 30(17): 328-335.
[5] 杨道兰, 汪建旭, 冯炜弘, 等. 花椰菜茎叶与玉米秸秆的混贮品质. 草业科学, 2014, 31(3): 551-557.
[6] 李树成, 黄晓辉, 王静, 等. 白花草木樨与玉米秸秆混合青贮的发酵品质及有毒成分分析. 草业科学, 2014, 31(2): 321-327.
[7] 黄晓辉, 李树成, 李东华, 等. 苦豆子和玉米秸秆的混合青贮. 草业科学, 2013, 30(10): 1633-1639.
[8] 詹发强, 包慧芳, 崔卫东, 等. 玉米青贮过程中乳酸菌动态变化. 微生物学通报, 2010, 37(6): 834-838.
[11] 司丙文, 王宗礼, 孙启忠, 等. 尖叶胡枝子青贮微生物数量变化及发酵特性. 草业科学, 2012, 29(4): 650-657.
[13] 杨杨, 石超, 郭旭生. 高寒草甸魏斯氏乳酸菌的分离鉴定及理化特性研究. 草业学报, 2014, 23(1):266-275.
[14] 司丙文, 王宗礼, 孙启忠, 等. 山竹岩黄芪青贮中优质乳酸菌的分离和鉴定. 草地学报, 2012, 20(1): 166-170.
[18] 郭兴华, 凌代文. 乳酸细菌现代研究实验技术[M]. 北京: 科学出版社, 2013: 285-286.
[21] 徐春城. 现代青贮理论与技术[M]. 北京: 科学出版社, 2013.
[22] 王晓娟, 杨阳, 张晓强, 等. 木质素与生物燃料生产: 降低含量或解除束缚. 中国农业科学, 2015, 48(2): 229-240.
[23] 刘晶晶, 高丽娟, 师建芳, 等. 乳酸菌复合系和植物乳杆菌提高柳枝稷青贮效果. 农业工程学报, 2015, 31(9): 295-302.
[25] 葛剑, 杨翠军, 杨志敏, 等. 紫花苜蓿和裸燕麦混贮发酵品质和营养成分分析. 草业学报, 2015, 24(4): 104-113.
[26] 蒋慧, 方雷, 周小玲, 等. 添加初花期骆驼刺改善苜蓿青贮品质. 农业工程学报, 2014, 30(17): 328-335.