Optimization of culture conditions of a biocontrol agent Bacillus mojavensis ZA1 to antagonize Phoma foveata

doi:10.11686/cyxb2015142

Abstract

Abstract: This work aimed to identify optimum fermentation shaker-flask culture conditions for Bacillus mojavensis strain ZA1. Various single culture factors were combined according to a Box-Behnken design. The optimum ratio of ingredients for the culture medium for ZA1was found to be: 14.25 g ammonium chloride, 19 g corn flour, and 237 g potato, in 1000 mL distilled water. The optimum cultivation conditions were pH 7.7, temperature 28℃, and shaken at 180 r/min for 36 h. Under these conditions, the viable count of B. mojavensis strain ZA1 was 4.12×10¹⁰ colony-forming units per mL (CFU/mL). Further testing using the central composite design method in a 10 L fermentation tank found the optimum for dissolved oxygen level and shaker rotation speed to be 60% saturation and 180 r/min respectively. Under these conditions, when fermentation time was 36 h in the fermentation tank, the viable count of B. mojavensis strain ZA1 reached 1.59×10¹¹ CFU/mL. The results of this study lay a solid foundation for the development of methodology for large scale production of biocontrol agents.

FENG Zhong-Hong, CHANG Tao, YANG Cheng-De, XUE Li, LI Xu, YANG Xiao-Li. Optimization of culture conditions of a biocontrol agent Bacillus mojavensis ZA1 to antagonize Phoma foveata[J]. Acta Prataculturae Sinica, 2016, 25(2): 77-86.

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