Physiological and biochemical responses of Medicago sativa seed to cobalt stress

doi:10.11686/cyxb2015148

Abstract

Abstract: The aim of this study was to investigate the mechanism of cobalt (Co) stress damage on Medicago sativa as experiment materials, The effects of different concentrations (0, 0.25, 0.50, 1.00, 2.50, 5.00 mmol/L CoCl₂) of Co on M. sativa (cv. Gannong No.3) seed germination and seedling physiological and biochemical characteristics were assessed. The results indicate that Co stress significantly inhibited seed germination and seedling growth. Seed germination potential, germination rate, germination index, vigor index, seedling sprout length, radicle length, root activity and dry weight were significantly reduced with increasing cobalt concentration. The inhibitory effects of Co stress on germination potential was higher than that of cobalt stress on germination rate, and the inhibitory effects of Co stress on radical growth was higher than that on the plumule. Co significantly reduced the soluble protein content, soluble sugar content, proteolytic enzyme activity, superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and catalase (CAT) in seedling leaves. However free proline content increased dramatically at lower Co concentrations but declined at higher concentrations. At higher Co concentrations, O2·- (superoxide anion radical), OH^· (hydroxyl radical), H₂O₂ and malondialdehyde increased significantly. This illustrates that under high Co stress, the seedling antioxidant system activity and active oxygen scavenging ability of M. sativa decreased, inhibiting M. sativa seed germination and seedling growth.

ZHANG Hu, KOU Jiang-Tao, SHI Shang-Li. Physiological and biochemical responses of Medicago sativa seed to cobalt stress[J]. Acta Prataculturae Sinica, 2015, 24(9): 146-153.

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