The genetic diversity of 9 populations of dry-desert Agropyron mongolicum collected in northern China

doi:10.11686/cyxb2015219

Abstract

Abstract: Agropyron mongolicum is one of the dominant species in desert steppe across Eurasia, due primarily to its cold and drought resistance. In this study, we analyzed chromosome polymorphism and DNA polymorphism in 9 populations of A. mongolicum in northern China. The chromosome number of the 9 populations was 7. Cells were diploid and showed no polymorphism in chromosome ploidy. A total of 138 pairs of wheat SSR primers were amplified and analyzed. A total of 21 primer pairs were amplified with specific fragments. The screening rate of SSR primers was 15.2%. A total of 119 specific bands were amplified: specificity was 5.6 and the polymorphism of DNA was rich. POPGEN 32 software was used to calculate the genetic diversity of the 9 populations of A. mongolicum. Population P₈ was found to have the least level of diversity, while P₃ had the highest. AMOVA software was used to analyze genetic differentiation, indicating that genetic differences come mainly from individuals in the populations. The UPGMA method was used for a cluster analysis of the 9 populations. When the genetic similarity coefficient is 0.80, the materials tested divided into three groups: P₁-P₆, P₇-P₈ and P₉. This paper lays the foundation for the development and utilization of new varieties of A. mongolicum.

LI Xiao-Quan, GAO You-Han, LIU Yang, SUO Pei-Fen, Han Bing. The genetic diversity of 9 populations of dry-desert Agropyron mongolicum collected in northern China[J]. Acta Prataculturae Sinica, 2016, 25(3): 77-85.

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