Screening of cellulose-degrading bacteria and evaluation of silage performance of Pennisetum

doi:10.11686/cyxb2015290

Abstract

Abstract: Cellulose-degrading bacteria play important roles in improving forage quality and increasing forage utilization. In this study, a variety of cellulose-degrading bacteria were screened to identify strains with high enzyme activity. Three strains (designated as No.11, No.21, and No.36) were selected from the screening experiment and used to produce silage from hybrid Pennisetum. The four treatment groups consisted of treatment 1 (control, CK; hybrid Pennisetum without broth), and treatments 2, 3, and 4 (hybrid Pennisetum with broth of strain No.11, 21, and 36, respectively). The addition of cellulose-degrading bacteria into silage of hybrid Pennisetum increased the lactic acid content. The lactic acid contents of treatments 2, 3 and 4 were 19.42% (P<0.05), 38.35% (P<0.05), 4.85% (P>0.05) higher, respectively, than that in CK. The neutral detergent fiber (NDF), acid detergent fiber (ADF), and hemicellulose (HC) contents of silage were lower in treatments 2, 3, and 4 than in CK and in the Pennisetum mixtures before ensiling. The silage produced in treatment 3 had the lowest NDF, ADF, and HC contents. The three strains screened from many cellulose-degrading bacteria improved silage quality, and degraded cellulose to different degrees. Among the three strains, No.21 showed the best performance in degrading cellulose of hybrid Pennisetum and produced silage with the best nutritional quality.

HUANG Qin-Lou, ZHONG Zhen-Mei, HUANG Xiu-Sheng, CHEN Zhong-Dian, FENG De-Qing, XIA You-Guo. Screening of cellulose-degrading bacteria and evaluation of silage performance of Pennisetum[J]. Acta Prataculturae Sinica, 2016, 25(4): 197-203.

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