ResistanceevaluationofdifferentalfalfavarietiestoFusariumsporotrichioides

doi:10.11686/cyxb2015331

Abstract

Abstract: The present study evaluated the resistance of 30 alfalfa varieties to Fusariumsporotrichioides by inoculation of 45 d old seedlings in a greenhouse. Resistant and susceptible varieties were selected based on the root disease index after 10 days of inoculation and were further evaluated by inoculation pathogen and measuring enzymes activities after 4,8 and 12 hours inoculation. The results showed that there were four resistant varieties: Jinnan, Xunlu, PG Saite and Sulian No.2, which together accounted for 13.3%. There were seven highly sensitive varieties: Xinjiangdaye, Zhongmu No.1, Longzhong, Beijiang, Huangguan, Gansu and Salansi, which together accounted for 23.3%. The remaining varieties were all sensitive and together accounted for 63.4%. There were no resistant or immune varieties. Measurement of enzyme activities showed highly significant variations in CAT, SOD and PAL activities among the different varieties, for both the inoculated samples and for the controls without inoculation. Enzyme activities in inoculated plants were higher than those of control plants and enzyme activities in the resistant varieties were higher than those of susceptible varieties. Enzyme activity increased after 4-8 d inoculation and decreased after 8-12 d inoculation. CAT activity of the inoculated plants significantly increased compared to the control, while SOD and PAL activities did not significantly increase.

PAN Long-Qi, ZHANG Li, TIAN Jin-Shan, YUAN Qing-Hua, WANG Yu, MIAO Li-Hong. ResistanceevaluationofdifferentalfalfavarietiestoFusariumsporotrichioides[J]. Acta Prataculturae Sinica, 2016, 25(5): 95-101.

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