Construction of a genetic linkage map for tetraploid hybrid wheatgrass using a SSR molecular marker

doi:10.11686/cyxb2015410

Abstract

Abstract: To establish a genetic linkage map in tetraploid hybrid wheatgrass genetic mapping was conducted using a simple sequence repeats (SSR) molecular marker technique with ‘Joinmap’ 4.0 software. 347 individuals from the F₂ segregating population and their parents were utilized, this helped lay the foundation for further study of marker-assisted breeding, and quantitative trait locus (QTL) location of important traits in wheatgrass, such as disease resistance and yield. Thirty optimal primers with clear, stable and high polymorphic bands were screened from 256 tested SSR primers. A total of 224 SSR loci were obtained from polymerase chain reaction (PCR) amplification with an average of 7.47 loci per primer, of which 185 were polymorphic loci, accounting for 82.6% of all loci. Segregation distortion analysis showed that a total of 24 loci were distorted, accounted for 13.0% of all (185) polymorphic loci, which met the requirement of <30% segregation distortion for plant genetic mapping. These polymorphic loci would be useful for genetic mapping. In this study, a molecular genetic linkage map of tetraploid hybrid wheatgrass was constructed which contained 185 loci and 14 linkage groups with length ranging from 123.0 to 202.6 cM. The longest and the shortest linkage group were LG4 and LG12, respectively. The average length of each linkage group was 167.32 cM, total length of the genome was 2342.5 cM and the average marker distance was 12.66 cM.

JIANG Zhi-Yan, YU Xiao-Xia, YU Zhuo, ZHANG Zhi-Cheng, SHI Yue, JIANG Chao. Construction of a genetic linkage map for tetraploid hybrid wheatgrass using a SSR molecular marker[J]. Acta Prataculturae Sinica, 2016, 25(6): 94-101.

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