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Acta Prataculturae Sinica ›› 2016, Vol. 25 ›› Issue (2): 27-36.DOI: 10.11686/cyxb2015422

• Orginal Article • Previous Articles     Next Articles

The effect of compound bio-fertilizers on soil physical and chemical properties and soil enzyme activity in Leymus chinensis steppe

QUAN Guo-Ling1,2, XIE Kai-Yun1, TONG Zong-Yong1, LI Xiang-Lin1,2,*, WAN Li-Qiang1, BI Shu-Yi1, WAN Xiu-Fu1   

  1. 1.Institute of Animal Sciences, Chinese Academy of Agriculture Sciences, Beijing 100193, China;
    2.The State Key Laboratory of Grassland Agroecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
  • Received:2015-09-08 Online:2016-02-20 Published:2016-02-20

Abstract: Compound bio-fertilizers have recently been developed to improve the performance of bio-fertilizers. These compounds are a mix of inorganic chemical, organic and bio-fertilizers. In this study, we investigated the effect on Leymus chinensis steppe soil fertility of three kinds of compound bio-fertilizer: humic acid, molasses fermentation and alginic acid. We also measured the effects of a mixed application of these three compounds. Results showed that compared to the control there was a significant reduction in total phosphorus in surface soils under the molasses fermentation and three compound mix treatments. Soil organic matter increased significantly, with these increases being higher for the three compound mix than for the humic acid and molasses fermentation treatments. The other soil physical and chemical properties measured showed no significant change under each treatment. Soil enzyme activities were significantly affected. Compared with the control, hydrogen peroxide enzyme activity in the 0-10 cm soil layer was significantly reduced in all treatments except for the humic acid compound. Phosphatase activity increased significantly in all cases except for the alginic acid treatment. Urease activity was reduced significantly in the humic acid and molasses fermentation treatments. Invertase activity did not change significantly. In 10-20 cm soil layer, hydrogen peroxide enzyme activity increased significantly in the humic acid treatment while it significantly reduced under the other treatments. Invertase activity did not change with the three compound mix but reduced significantly under the remaining three treatments. Urease activity decreased only with humic acid treatment, while phosphatase activity did not significantly change in any of the treatments. Hydrogen peroxide enzyme activity and soil pH were significantly positively correlated, while urease and total nitrogen were significantly negatively correlated. Phosphatase and total phosphorus had a significant negative correlation in topsoil. Invertase activity was significantly positively correlated with organic matter, and was significantly negatively correlated with soil moisture content in the lower soils. In general, soil enzyme activity decreased with increasing depth of soil profile.