The effect of acarbose addition on rumen and hindgut fermentation in dairy cows fed high-concentrate diets

doi:10.11686/cyxb2015431

Abstract

Abstract: In this study, three cannulated Holstein cows (590±48 kg liveweight) were used to investigate the effect of acarbose (glucosidase inhibitor) addition (0, 0.5 and 1.0 g/d) on ruminal and hindgut fermentation characteristics in a 3×3 Latin square design. The results showed that acarbose addition decreased the propionate concentration in rumen fluid (P<0.05), increased the ratio of acetate to propionate (P<0.05) but did not affect the concentrations of lactate, acetate, isobutyrate, butyrate, isovalerate, valerate, total volatile fatty acid, ammonia-nitrogen, and pH value compared with the control (P>0.05). Acarbose addition decreased the pH value and ammonia-nitrogen levels (P<0.05) and increased the concentrations of lactate, butyrate, isovalerate (P<0.05) in feces, but no differences were observed in the concentration of acetate, propionate, isobutyrate, valerate, total volatile fatty acid and the ratio of acetate to propionate among the three groups. In general, our data revealed that acarbose addition may not be useful for preventing the occurrence of ruminal acidosis in the long term. Additionally, acarbose addition may increase hindgut fermentation and produce detrimental effects on the hindgut health of dairy cattle.

YIN Yu-Yang, LIU Yu-Jie, ZHANG Rui-Yang, ZHU Wei-Yun, MAO Sheng-Yong. The effect of acarbose addition on rumen and hindgut fermentation in dairy cows fed high-concentrate diets[J]. Acta Prataculturae Sinica, 2016, 25(6): 198-203.

References

[1] Krehbiel C R, Britton R A, Harmon D L, et al . The effects of ruminal acidosis on volatile fatty acids absorption and plasma activities of pancreatic enzymes in lambs. Journal of Animal Science, 1995, 73(10): 3111-3121.
[2] Garrett E F, Nordlund K V, Goodger W J, et al . A cross-sectional field study investigating the effect of periparturient dietary management on ruminal pH in early lactation dairy cows. Journal of Dairy Science, 1997, 80(Suppl.1): 169.
[3] Nordlund K V, Cook N B, Oetzel G R. Investigation strategies for laminitis problem herds.Journal of Dairy Science, 2004, 87: E27-E35.
[4] Greenough P R, Vermunt J J, McKinnon J J, et al . Laminitis like changes in the claws of feedlot cattle. Canadian Veterinary Journal, 1990, 31: 202-208.
[5] Nordlund K V, Garret E F. Rumenocentesis: A technique for collecting rumen fluid for the diagnosis of subacute rumen acidosis in dairy herds. The Bovine Practitioner, 1994, 28: 109-112.
[6] Garret E. Subacute rumen acidosis-clinical signs and diagnosis in dairy herds. Large Animal Veterinarian, 1996, 11: 6-10.
[7] Plaizier J C, Krause D O, Gozho G N, et al . Subacute ruminal acidosis in dairy cows: The physiological causes, incidence and consequences. The Veterinary Journal, 2008, 176(1): 21-31.
[8] Kleen J L, Hooijer G A, Rehage J, et al . Subacute ruminal acidosis (SARA): A review. Journal of Veterinary Medicine Series A-Physiology Pathology Clinical Medicine, 2003, 50(8): 406-414.
[9] Long S, Li P, You L X. The prevention and control measures of subacute ruminal acidosis (SARA) in dairy herds. Feed Industry, 2010, 31(23): 61-64.
[10] Liu C J, Sun M J, Lv W L, et al . Effects of probiotics on feeding composite mid-lactation milk yield and milk components. China Feed, 2001, 1: 22-24.
[11] Callaway T R, Martin S A. Effects of organic acid and monensin treatment on in vitro mixed ruminal microorganism fermentation of cracked corn. Journal of Animal Science, 1996, 74: 1982-1989.
[12] McLaughlin C L, Thompson A, Sherington J, et al . Effect of acarbose on acute acidosis. Journal of Dairy Science, 2009, 92(6): 2758-2766.
[13] McLaughlin C L, Thompson K, Sherington J, et al . Effect of acarbose on milk yield and composition in early-lactation dairy cattle fed a ration to induce subacute ruminal acidosis. Journal of Dairy Science, 2009, 92: 4481-4488.
[14] Mao S Y, He W B, Zhu W Y. Effect of acarbose addition on acute and subacute rumen acidosis in an in vitro fermentation study. Acta Prataculturae Sinica, 2012, 21(6): 130-136.
[15] Yang G Y, Ren J, Liu J P. From the patient's point rigorously evaluated effect of acarbose in the treatment of diabetes. Chinese Journal of Drug Evaluation, 2012, 5: 375-380.
[16] Weathburn M W. Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry, 1967, 39: 791-794.
[17] Baker S B, Summerson W H. The colorimetric determination of lactic acid in biological material. Journal of Biological Chemistry, 1941, 38: 535-554.
[18] Qin W L. Determination of rumen volatile fatty acids by means of gas chromatography. Journal of Nanjing Agricultural College, 1982, 4: 110-116.
[19] Tajima K, Aminov R I, Nagamine T, et al . Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Applied and Environmental Microbiology, 2002, 67(6): 2766-2774.
[20] Kleen J L, Cannizzo C. Incidence, prevalence and impact of SARA in dairy herds. Animal Feed Science and Technology, 2012, 172(1-2): 4-8.
[21] Speight S M, Harmon D L. Batch culture evaluation of carbohydrase inhibitors to moderate rumen fermentation. Animal Feed Science and Technology, 2010, 155: 156-162.
[22] Speight S M, Harmon D L, Tricarico J M. Application of carbohydrase inhibitors to moderate rumen fermentation: Continuous culture evaluation. Journal of Dairy Science, 2007, 90(Suppl.1): 340.
[9] 龙森, 李鹏, 尤丽霞. 奶牛亚急性瘤胃酸中毒(SARA)的防治措施. 饲料工业, 2010, 31(23): 61-64.
[10] 刘彩娟, 孙满吉, 吕文龙, 等. 饲喂复合益生菌对泌乳中期奶牛产奶量及乳成分的影响. 中国饲料, 2011, 1: 22-24.
[14] 毛胜勇, 何文波, 朱伟云. 酸中毒条件下添加阿卡波糖对瘤胃微生物发酵的影响. 草业学报, 2012, 21(6): 130-136.
[15] 杨国彦, 任君, 刘建平. 从患者角度严格评价阿卡波糖在糖尿病治疗中的作用. 中国药物评价, 2012, 5: 375-380.
[18] 秦为琳. 应用气相色谱测定挥发性脂肪酸方法的研究改进. 南京农业大学学报, 1982, 5: 110-116.