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Acta Prataculturae Sinica ›› 2016, Vol. 25 ›› Issue (9): 152-160.DOI: 10.11686/cyxb2015511

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Effects of microecological agents on the fermentation quality, nutrition composition and in situ ruminal degradability of corn stalk silage

TAO Lian, FENG Wen-Xiao, WANG Yu-Rong, DIAO Qi-Yu*   

  1. Feed Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, China
  • Received:2015-11-10 Online:2016-09-20 Published:2016-09-20

Abstract: A study has been undertaken to investigate the effects of probiotics on the fermentation quality, nutrition composition and in situ ruminal degradability of corn stalk silage. Green corn stalk without ears (dry matter=42%-44%) was chopped to 2 cm lengths and then 1 kg of chopped corn stalk was packed with additives (zymin, fungus-preparation, and a mixture of the zymin and fungus-preparation) in polyethylene bags (24 cm×40 cm) using a vacuum packager. After 45 days of fermentation at room temperature, the corn stalk silage’s fermentation quality, nutrition composition and in situ ruminal degradability were analyzed using laboratory chemical methods and in situ technology. The results showed that, compared to the control, the pH value and ammonia nitrogen (NH3-N) content of the two lactobacillus (LAB1 and LAB2) treatments decreased (P<0.05), whereas contents of lactate acid (LA), organic matter (OM), water soluble carbohydrates (WSC), and the in situ ruminal degradability of DM, OM and crude protein (CP) increased significantly (P<0.05). The addition of lactobacillus increased WSC content (P<0.05) compared with the control and cellulase treatments. Compared with the control, the pH value, NH3-N content, neutral detergent fiber (NDF), acid detergent fiber (ADF), and cellulose content of the cellulase treatments (CE1 and CE2) significantly decreased (P<0.05). Furthermore, the acid detergent lignin (ADL) content had a decreasing trend (P>0.05). The mixture of cellulase and lactobacillus treatments (MCL1 and MCL2) had the lowest pH value and the highest in situ degradation of NDF and cellulose compared with other treatments (P<0.05). In addition, in situ degradation of ADL increased but did not reach significance (P>0.05). Compared with the control, the NDF, ADF and cellulose contents of the cellulase and lactobacillus mixture treatments significantly decreased (P<0.05). In conclusion, the application of cellulase and lactobacillus mixtures to the fermentation of corn stalk contributed to the breakdown of lignin-cellulose-hemicellulose composite structures and the improvement of fermentation quality, which consequently resulted into high nutrient digestibility and utilization efficiency. The MCL2 treatment had the best effect in this regard.