Identification and study on the effects of plant growth promoting rhizobacteria of Carex enervis

doi:10.11686/cyxb2016026

Abstract

Abstract: Six plant growth-promoting rhizobacteria were isolated from Carex enervis. We analyzed the six strains to evaluate their phosphate solubilization (by molybdenum blue colorimetry), nitrogen fixation (by the acetylene reduction assay), and hormone secretion (by high performance liquid chromatography). The two strains with the strongest P solubilization and N fixation abilities were identified by analyses of their 16S rDNA sequences. The results showed that the phosphate solubilization capacity of the six strains ranged from 298.17 to 554.67 mg/L (in strain TPRS3). Four strains were able to fix nitrogen, with nitrogenase activity ranging from 170.19 to 456.87 nmol C₂H₄/(mL·h) (in strain TPRS19), while strains TPRS3 and TPRS12 lacked nitrogenase activity. All six strains secreted hormones (indole acetic acid (IAA), ranging from 8.20 to 86.36 mg/L; gibberellin (GA₃) ranging from 26.36 to 135.90 mg/L; and trans-zeatin (t-Z), ranging from 9.55 to 141.68 mg/L). Strain TPRS19 secreted the largest amount of IAA (86.36 mg/L), strain TPRS5 secreted the largest amount of GA₃ (135.90 mg/L), and TPRS2 secreted the largest amount of t-Z (141.68 mg/L). Strains TPRS3, TPRS5, TPRS12, and TPRS19 could secrete all three hormones simultaneously. Strain TPRS5 was identified as Bacillus subtilis and TPRS19 was identified as Enterobacter cloacae. These strains have the potential for use as microbial fertilizers.

LIU Ting, YAO Tuo, CHEN Jian-Gang, MA Wen-Bin, LIU Huan, MA Cong-Yu, JIANG Yong-Mei. Identification and study on the effects of plant growth promoting rhizobacteria of Carex enervis[J]. Acta Prataculturae Sinica, 2016, 25(12): 130-139.

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