Abstract: Based on the known sequence of GGPPS, which encodes geranylgeranyl pyrophosphate synthase, the GGPPS promoter sequence was amplified from the root of the perennial herb Taraxacum kok-saghyz by thermal asymmetric interlaced PCR (TAIL-PCR) using nested specific primers. Analyses of the full-length 1131-bp fragment by PlantCare and PLACE software showed that the promoter sequence contained basic cis-acting elements, multiple organ-specific expression elements, and a number of stress-related cis-acting elements. The promoter sequence was designated as pTkGGPPS (GenBank: KT901796). The plant expression vector pCAMBIA1304-pTkGGPPS-GFP was constructed using this sequence to replace the CaMV 35S promoter sequence in the plasmid of pCAMBIA1304 with GFP as the reporter gene. The construct was transformed into onion epidermal cells using Agrobacterium tumefaciens, and the promoter successfully drove expression of the GFP gene. The cloning and transient expression of the GGPPS gene promoter from T. kok-saghyz provides a reference for further studies on the mechanisms and tissue-specificity of rubber synthesis.