Biological characteristics of Laetisaria fuciformis and Limonomyces roseipellis and their responses to different fungicides

doi:10.11686/cyxb2016058

Abstract

Abstract: Red thread and pink patch, caused by the fungi Laetisaria fuciformis and Limonomyces roseipellis, respectively, are important diseases of many turfgrass species worldwide. In this study, we analyzed the biological characteristics of these two pathogens and evaluated their responses to 14 different fungicides. The results showed that L. fuciformis and Li. roseipellis grew well at pHs ranging from 5 to 7, and the optimal pH value was 6. The hyphae of both pathogens lost viability in agar plugs that were incubated at 50 ℃ for 10 min. The optimal carbon and nitrogen sources for L. fuciformis and Li. roseipellis were soluble starch and L-glutamine, respectively. The optimal medium for L. fuciformis was oatmeal agar (OMA) and that for Li. roseipellis was potato dextrose agar (PDA) or potato sucrose agar (PSA). Among the 14 fungicides, diniconazole had the strongest inhibitory effects on L. fuciformis and Li. roseipellis with EC₅₀ values of 0.113 mg/L and 0.282 mg/L, respectively. The fungicide with the weakest inhibitory effects was 15% metalaxyl·10% propamocarb, with EC₅₀ values of 121.522 and 214.532 mg/L for L. fuciformis and Li. roseipellis, respectively. The results provide a scientific basis for understanding the basic biology of these pathogens, and may be useful for the development of new management strategies.

ZHANG Wu, HU Mei-Jiao, GAO Zhao-Yin, LI Min, LIU Guo-Dao, NAN Zhi-Biao. Biological characteristics of Laetisaria fuciformis and Limonomyces roseipellis and their responses to different fungicides[J]. Acta Prataculturae Sinica, 2016, 25(12): 140-149.

References

[1] Xu B L. The Lawn Protection[M]. Beijing: Chinese Forestry Press, 2013.
[2] Nan Z B, Li C J. The directory of forage grasses diseases in China. Pratacultural Science, 1994, 13(Supplementary issue): 1-160.
[3] Lv C, Luo L, Hsiang T. First report of dollar spot of seashore paspalum ( Paspalum vaginatum ) caused by Sclerotinia homoeocarpa in South China. Plant Disease, 2010, 94(3): 373.
[4] Zhang W, Nan Z B, Liu G D, et al . First report of leaf and sheath spot caused by Waitea circinat avar. zeae on Paspalum vaginatum and Zoysia tenuifolia in China. Plant Disease, 2014, 98(10): 1436.
[5] Zhang W, Liu G D, Nan Z B. Identification and biological charactertistics of Sclerotinia homoeoparpa causing dollar spot on 4 warm-season turfgrass. Acta Prataculturae Sinica, 2015, 24(1): 124-131.
[6] Zhang W, Nan Z B, Tian P, et al . Microdochium paspali , a new species causing seashore paspalum disease in south China. Mycologia, 2015, 107(1): 80-89.
[7] Couch H B. Disease of Turfgrass[M]. Malabar, F L: Krieger Publishing Company, 1995.
[8] Kaplan J D, Jackson N. Red thread and pink patch diseases of turfgrasses. Plant Disease, 1983, 67(2): 159-162.
[9] Smiley R W, Dernoeden P H, Clarke B B. Compendium of Turf grass Diseases[M]. St. Paul: American Phytopathological Society (APS Press), 2005.
[10] Stalpers J A, Loerakker W M. Laetisaria and Limonomyces species (Corticiaceae) causing pink diseases in turfgrasses. Canadian Journal of Botany, 1982, 60(5): 529-537.
[11] Maccaroni M, Corazza L, Buonaurio R, et al . Occurrence of pink patch of perennial ryegrass caused by Limonomyces roseipellis in Italy. Plant Disease, 2002, 86(1): 74.
[12] Burdsall H H. Laetisaria (Aphyllophorales, Corticiaceae), a new genus for the teleomorph of Isaria fuciformis . Transaction of the British Mycological Society, 1979, 72(3): 419-422.
[13] Zhang W, Nan Z B, Liu G D. First report of Laetisaria fuciformis causing red thread on seashore paspalum ( Paspalum vaginatum ) in south China. Plant Disease, 2012, 96(9): 1374.
[14] Zhang W, Nan Z B, Liu G D. First report of Limonomyces roseipellis causing pink patch on bermudagrass in south China. Plant Disease, 2013, 97(4): 561.
[15] Zhang W, Hu M J, Liu G D, et al . Investigation and characterization of red thread and pink patch on warm-season turfgrasses in Hainan Province, tropical China. European Journal of Plant Pathology, 2015, 141(2): 311-325.
[16] Li Y Z, Nan Z B. Nutritional study on Embellisia astragali , a fungal pathogen of milk vetch ( Astragalus adsurgens ). Antonie van Leeuwenhoek, 2009, 95(3): 275-284.
[17] Gould C J, Goss R L, Vassey W E. Fungicidal tests for control of Corticium red thread in the Pacific North West. Journal of the Sports Turf Research Institute, 1976, 51: 62-66.
[18] Koch P L, Grau C R, Jo Y K, et al . Thiophanate-methyl and propiconazole sensitivity in Sclerotinia homoeocarpa populations from golf courses in Wisconsin and Massachusetts. Plant Disease, 2009, 93(1): 100-105.
[19] Young J R, Tomaso-Peterson M, De la Cerda K, et al . Two mutations in β-tubulin 2 gene associated with thiophanate-methyl resistance in Colletotrichum cereale isolates from creeping bentgrass in Mississippi and Alabama. Plant Disease, 2010, 94(2): 207-212.
[20] Wong F P, De la Cerda K A, Hernandez-Martinez R, et al . Detection and characterization of benzimidazole resistance in California populations of Colletotrichum cereale . Plant Disease, 2008, 92(2): 239-246.
[21] Putman A I, Jung G, Kaminski J E. Geographic distribution of fungicide-insensitive Sclerotinia homoeocarpa isolates from golf courses in the northeastern United States. Plant Disease, 2010, 94(2): 186-195.
[22] Warren C G, Sanders P, Cole H J, et al . Relative fitness of benzimidazole- and cadmium-tolerant populations of Sclerotinia homoeocarpa in the absence and presence of fungicides. Phytopathology, 1977, 67: 704-708.
[23] Warren C G, Sanders P, Cole H. Sclerotinia homoeocarpa tolerance to benzimidazole configuration fungicides. Phytopathology, 1974, 64: 1139-1142.
[24] Detweiler A R, Vargas Jr J M, Danneberger T K. Resistance of Sclerotinia homoeocarpa to iprodione and benomyl. Plant Disease, 1983, 67(6): 627-630.
[25] Wang J H, Duo D. Effect of paclobutrazol on drought resistance of six turfgrass cultivars during the seeding stage. Acta Prataculturae Sinica, 2014, 23(6): 253-258.
[26] Liu Q, Yao T, Ma H L. Combined effects of bio-fertilizer and citric acid on turf quality and soil biology on a calcareous soil. Acta Prataculturae Sinica, 2014, 23(5): 223-230.
[27] Li X Y, Wang Y R, Jia C Z. Effect of urea fertilizer on growth and performance of Cleistogenes songorica turfgrass. Acta Prataculturae Sinica, 2014, 23(6): 136-141.
[28] Woolhouse A R. Assessment of perennial ryegrass cultivars for susceptibility to red thread disease. Journal of the Sports Turf Research Institute, 1996, 67: 147-152.
[29] Raikes C, Leep N W, Canaway P M. The effect of red thread ( Laetisaria fuciformis ) incidence on wear tolerance of monostands of perennial ryegrass ( Lolium perenne ) cultivars under three nitrogen. Journal of the Sports Turf Research Institute, 1996, 72: 61-66.
[30] Cahill J V, Murray J J, Dernoeden P H. Interrelationships between fertility and red thread fungal disease of turf grasses. Plant Disease, 1983, 67(10): 1080-1083.
[31] Tredway L P, Soika M D, Clarke B B. Red thread development in perennial ryegrass in response to nitrogen, phosphorus, and potassium fertilizer applications. International Turfgrass Society Research Journal, 2001, 9: 715-721.
[1] 徐秉良. 草坪保护学[M]. 北京: 中国林业出版社, 2013.
[2] 南志标, 李春杰. 中国牧草真菌病害名录. 草业科学, 1994, 13(增刊): 1-160.
[5] 章武, 刘国道, 南志标. 4种暖季型草坪草币斑病病原菌鉴定及其生物学特性. 草业学报, 2015, 24(1): 124-131.
[25] 王竞红, 多多. 多效唑对6种草坪草苗期抗旱性影响的研究. 草业学报, 2014, 23(6): 253-258.
[26] 刘强, 姚拓, 马晖玲. 菌肥与柠檬酸互作对石灰性土壤生物学特性及草坪质量的影响. 草业学报, 2014, 23(5): 223-230.
[27] 李欣勇, 王彦荣, 贾存智. 施尿素对无芒隐子草草坪生长特性的影响. 草业学报, 2014, 23(6): 136-141.