Identification of soybean growth-promoting rhizobacteria and their effects on the growth and quality of Glycine max and Lotus corniculatus

doi:10.11686/cyxb2016063

Abstract

Abstract: In order to screen combinations of rhizobacteria which have growth-promoting and quality-improving effects on local leguminous crops, phosphorus-solubilizing bacteria were isolated from soybean rhizospheric soils in the Bijie Region of Guizhou Province and rhizobium strains were isolated from soybean root nodules. Phosphorus-solubilizing strains with ratios of the diameter of the phosphorus-solubilizing halo to colony diameter of more than 2.20 were tested for their abilities of phosphorus-solubilizing, 3-indoleacetic acid (IAA)-secreting, organic acid and alkali production. Four superior phosphate-solubilizing strains were selected. For the isolated rhizobium strains, two highly efficient strains were screened out by means of back-inoculation and growth-promoting effect tests on soybean tube seedlings. The six selected strains were tested for antagonistic reactions and three types of bacterial suspension were prepared: phosphate dissolving only, rhizobia only and a mix of the two. These were immediately inoculated into Glycine max and Lotus corniculatus in pots to test their growth-promoting effects. The results showed that, compared to control, the rhizobia only inoculants had no effect on the plant height of G. max whereas the other two treatments could significantly increase height. All three treatments had significant promoting effects on G. max stem diameter, biomass, number of bean pods, weight of pods, seeds per pod and single seed weight. Among the three treatments, the mix of phosphate dissolving and rhizobia inoculants showed the best growth effects on soybean seedling plant height, stem diameter, aboveground biomass and underground biomass (respectively 21.26%, 40.79%, 15.88% and 42.19% higher than control). With regard to L. corniculatus, all three treatments could improve first and second harvest plant height, aboveground biomass, total nitrogen content, total phosphorus content and crude protein content. Again, the effects of the mixed treatment of phosphate dissolving with rhizobia inoculants were the highest (respectively 20.82%, 54.88%, 106.14%, 148.78%, 19.34%, 61.88% and 19.34% higher than control). The results thus indicate that combining phosphate-solubilizing with rhizobium bacteria strains produces a favourable interaction effect.

ZENG Qing-Fei, WANG Qian, LU Rui-Xia, LIU Zheng-Shu, WU Jia-Hai, WANG Xiao-Li. Identification of soybean growth-promoting rhizobacteria and their effects on the growth and quality of Glycine max and Lotus corniculatus[J]. Acta Prataculturae Sinica, 2017, 26(1): 99-111.

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