Allelopathic effects of leaf-stem litter water aqueous extracts of three plant species on tobacco seedlings

doi:10.11686/cyxb2016104

Abstract

Abstract: Tobacco, and the two Chinese herbal medicine crops Scrophularia ningpoensis and Chuanminshen violaceum, all present husbandry difficulties under continuous cropping systems, and allelopathic effects are considered to be a significant contributing factor. There have been relatively few studies of auto-allelopathy in tobacco crops or of allelopathy between tobacco and the Chinese herbal medicine crops S. ningpoensis and C. violaceum. To investigate problems arising in continuous cropping of tobacco, a study was conducted on leachate effects on seedlings. Flue-cured tobacco K126 and 1 year old leaf and stem litter were used to prepare aqueous extracts of leachates of tobacco (LeT), and leaf and stem litter were also used to prepare aqueous extracts of S. ningpoensis (LeS), and C. violaceum (LeC), with pure water as control. Treatment concentrations were 0.01, 0.02, 0.05, 0.10 mg/L. LeT had an inhibitory effect on seedlings below ground and above ground growth, as indicated by elevated antioxidant enzyme activity. For LeS the highest concentration of 0.10 mg/L showed significant inhibition, while the lowest concentration of 0.01 mg/L had a stimulatory effect. Meanwhile, LeC had little impact on tobacco seedlings, or tended to induce positive growth promotion effects. Following application of the aqueous extracts of S. ningpoensis and C. violaceum, SOD, POD, CAT activities were increased with increasing concentration of the extracts, and soluble protein, initial chlorophyll fluorescence (F₀), the maximum enhanced fluorescence (F_m), variable fluorescence (F_v), PSⅡ potential photochemical activity (F_v/F₀), and PSⅡ maximum photochemical efficiency (F_v/F_m), also showed better adaptation. Therefore, S. ningpoensis and C. violaceum may be an alternative rotation or intercropping plant species for tobacco, and intercropping may help ease husbandry problems encountered in continuous cropping of tobacco.

ZHAO Xin-Mei, WANG Jun, MO Jing-Jing, YANG Shui-Ping, WEN Ming-Xia, ZHANG Xue, ZHAO Jian, CHEN Da-Xia, JIANG Wei. Allelopathic effects of leaf-stem litter water aqueous extracts of three plant species on tobacco seedlings[J]. Acta Prataculturae Sinica, 2016, 25(9): 37-45.

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