Antioxidant enzyme activity and gene expression in creeping bentgrass under salt stress

doi:10.11686/cyxb2016107

Abstract

Abstract: The aim of this research was to investigate the antioxidant enzyme activity and gene expression mechanisms in creeping bentgrass (Agrostis stolonifera) under salt stress. Two different salt-resistant creeping bentgrass cultivars, Penncross and SeasideⅡ, were treated with 0 (CK) and 200 mmol/L NaCl solutions. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POD) were measured. The qRT-PCR primers for the CytSOD, FeSOD, CAT, APX and POD genes of creeping bentgrass were designed using partial gene segments, which were identified with homology cloning techniques. The gene expression levels of CytSOD, FeSOD, CAT, APX, and POD were measured and the activities of antioxidant enzymes and gene expression of the two different salt-resistant creeping bentgrass cultivars were analyzed. Salt stress had different effects on antioxidase activities and gene expressions on the two creeping bentgrass cultivars. At the beginning of salt stress, SOD activity of salt-sensitive Penncross was higher than that of salt-resistant SeasideⅡ. However, in the later stages of the treatment, the activity of Penncross SOD declined dramatically while the activity of SeasideⅡ SOD declined slowly and tended to be relatively stable, during which the activity of SeasideⅡ SOD was significantly higher than Penncross. The activities of CAT, APX and POD increased with salt treatment. The CAT and APX activities of Penncross were lower than those of SeasideⅡ, while POD activity was not different. Under the salt treatment, CytSOD, CAT, APX and POD expression of Penncross increased while FeSOD expression decreased. For SeasideⅡ, gene expression of the five antioxidases increased; CytSOD, FeSOD, CAT and APX gene expression were significantly higher in SeasideⅡ than Penncross, but POD was not different. The relative expression levels of CytCu/ZnSOD, CAT, APX and POD genes changed consistently with enzyme activities, but the relative expression level of the FeSOD gene in Penncross was not consistent with enzyme activity.

XIAO Guo-Zeng, TENG Ke, LI Lin-Jie, CHAO Yue-Hui, HAN Lie-Bao. Antioxidant enzyme activity and gene expression in creeping bentgrass under salt stress[J]. Acta Prataculturae Sinica, 2016, 25(9): 74-82.

References

[1] Dean D E, Devitt D A, Verhick L S, et al . Turfgrass quality, growth, and water use influenced by salinity and water stress. Agronomy Journal, 1996, 88(5): 844-849.
[2] Morris R L. Water quality changes in golf course irrigation ponds transitioning to reuse water. Hortscience A Publication of the American Society for Horticultural Science, 2005, 40(7): 2151-2156.
[3] Qian Y L, Mecham B. Long-term effects of recycled wastewater irrigation on soil chemical properties on golf course fairways. Agronomy Journal, 2005, 97(3): 717-721.
[4] Zhu J K. Plant salt tolerance. Trends in Plant Science, 2001, 6(2): 66-71.
[5] Zhu J K. Regulation of ion homeostasis under salt stress. Current Opinion in Plant Biology, 2003, 6(5): 441-445.
[6] Barry H. Reactive species and antioxidants redox biology is a fundamental theme of aerobic life. Plant Physiology, 2006, 141(2): 312-322.
[7] Jiang M, Zhang J. Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize [ Zea mays ] seedlings. Plant & Cell Physiology, 2001, 42(11): 1265-1273.
[8] Liu Y J, Zhang L, Tian X Y, et al . The effects of salt stress on endogenous hormones, NADKase and Ca 2+ -ATPase in leaves of Puccinellia chinampoensis seedlings. Pratacultural Science, 2008, 25(4): 51-54.
[9] Bethke P C, Jones R L. Cell death of barley aleurone protoplasts is mediated by reactive oxygen species. Plant Journal, 2001, 25(1): 19-29.
[10] Demidchik V. Mechanisms of oxidative stress in plants: From classical chemistry to cell biology. Environmental and Experimental Botany, 2015, 109: 212-228.
[11] Scandalios J G. Oxygen stress and superoxide dismutases. Plant Physiology, 1993, 101(1): 7-12.
[12] Fan R P, Zhou Q, Zhou B, et al . Effects of salinization stress on growth and the antioxidant system of tall fescue. Acta Prataculturae Sinica, 2012, 21(1): 112-117.
[13] Elstner E F. Oxygen activation and oxygen toxicity. Annual Review of Plant Physiology, 2003, 33(1): 73-96.
[14] Bowler C, And M V M, Inze D. Superoxide dismutase and stress tolerance. Annual Review of Plant Physiology & Plant Molecular Biology, 1992, 43(4): 83-116.
[15] Wang Y, Gao P, Huang M, et al . Effects of high temperature on the activity and expression of antioxidative enzymes in rice flag leaves during the flowering stage. Plant Science Journal, 2015, 33(3): 355-361.
[16] Zhang Y X, Jin L, Feng S S, et al . Effects of Cd on activity and gene expression of antioxidant enzymes in hyperaccumulator Solanum nigrum L. Journal of Graduate University of Chinese Academy of Sciences, 2013, 30(1): 11-17.
[17] Zhao S C, Sun J W, Ma Y Z, et al . Effects of cadmium on reactive oxygen species metabolism, activities and gene expressions of superoxide dismutase and catalase in maize ( Zea mays ) seedling. Scientia Agricultura Sinica, 2008, 41(10): 3025-3032.
[18] Liang G Q, Sun J W, Zhou W, et al . Effects of calcium on activities and gene expressions of superoxide dismutase and catalas in apple ( Malus pumila Mill.) fruits. Plant Nutrition and Fertilizer Science, 2011, 17(2): 438-444.
[19] Tao H, Li H Y, Zhang X Z, et al . Toxic effect of NaCl on ion metabolism, antioxidative enzymes and gene expression of perennial ryegrass. Ecotoxicology & Environmental Safety, 2011, 74(7): 2050-2056.
[20] Wang N, Li H M. Research on salt tolerance of cool-season turf grass. Hubei Agricultural Sciences, 2011, 50(10): 2047-2051.
[21] Marcum K B. Salinity tolerance of 35 bentgrass cultivars. Hort Science, 2001, 36(2): 374.
[22] Hoagland D R, Arnon D I. The water-culture method for growing plants without soil. California Agricultural Experiment Station, 1950, 347(2): 32.
[23] Zhang J, Kirkham M B. Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytologist, 1996, 132(3): 361-373.
[24] Giannopolitis C N, Ries S K. Superoxide dismutase: I. Occurrence in higher plants. Plant Physiology, 1977, 59(2): 309-314.
[25] Bradford M M. A rapid method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, 72(s 1/2): 248-254.
[26] Amako K, Chen G X, Asada K. Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants. Plant & Cell Physiology, 1994, 35(3): 497-504.
[27] Wang L P, Sun J, Guo S R, et al . Salt stress tolerance of cucumber-grafted rootstocks. Chinese Journal of Applied Ecology, 2012, 23(5): 18-21.
[28] Lu F Q, Liu J L, Zhu R F, et al . Physiological responses and tolerance of four oat varieties to salt stress. Acta Prataculturae Sinica, 2015, 24(1): 183-189.
[29] Meng Y X, Wang S H, Wang J C, et al . Influences of CoCl 2 on the growth and seedling physiological indexes of Hordeum vulgare under NaCl stress. Acta Prataculturae Sinica, 2014, 23(3): 160-166.
[30] Asish Kumar P, Anath Bandhu D, Prasanna M. Defense potentials to NaCl in a mangrove, Bruguiera parviflora : differential changes of isoforms of some antioxidative enzymes. Journal of Plant Physiology, 2004, 161(5): 531-542.
[31] Duan J, Li J S, Kang Y. Exogenous spermidine affects polyamine metabolism in salinity-stressed cucumis sativus roots and enhances short-term salinity tolerance. Journal of Plant Physiology, 2008, 165(15): 1620-1635.
[32] Puyang X, An M, Han L, et al . Protective effect of spermidine on salt stress induced oxidative damage in two Kentucky bluegrass ( Poa pratensis L.) cultivars. Ecotoxicology & Environmental Safety, 2015, 117: 96-106.
[33] Nounjan N, Nghia P T, Theerakulpisut P. Exogenous proline and trehalose promote recovery of rice seedlings from salt-stress and differentially modulate antioxidant enzymes and expression of related genes. Journal of Plant Physiology, 2012, 169(6): 596-604.
[8] 刘延吉, 张蕾, 田晓艳, 等. 盐胁迫对碱茅幼苗叶片内源激素、NAD激酶及Ca 2+ -ATPase的效应. 草业科学, 2008, 25(4): 51-54.
[12] 樊瑞苹, 周琴, 周波, 等. 盐胁迫对高羊茅生长及抗氧化系统的影响. 草业学报, 2012, 21(1): 112-117.
[15] 王艳, 高鹏, 黄敏, 等. 高温对水稻开花期剑叶抗氧化酶活性及基因表达的影响. 植物科学学报, 2015, 33(3): 355-361.
[16] 张玉秀, 金玲, 冯珊珊, 等. 镉对镉超累积植物龙葵抗氧化酶活性及基因表达的影响. 中国科学院研究生院学报, 2013, 30(1): 11-17.
[17] 赵士诚, 孙静文, 马有志, 等. 镉对玉米幼苗活性氧代谢、超氧化物歧化酶和过氧化氢酶活性及其基因表达的影响. 中国农业科学, 2008, 41(10): 3025-3032.
[18] 梁国庆, 孙静文, 周卫, 等. 钙对苹果果实超氧化物歧化酶、过氧化氢酶活性及其基因表达的影响. 植物营养与肥料学报, 2011, 17(2): 438-444.
[20] 王娜, 李海梅. 冷季型草坪草耐盐性研究. 湖北农业科学, 2011, 50(10): 2047-2051.
[27] 王丽萍, 孙锦, 郭世荣, 等. 黄瓜砧用白籽南瓜对不同盐胁迫的耐性评价. 应用生态学报, 2012, 23(5): 18-21.
[28] 刘凤歧, 刘杰淋, 朱瑞芬, 等. 4种燕麦对NaCl胁迫的生理响应及耐盐性评价. 草业学报, 2015, 24(1): 183-189.
[29] 孟亚雄, 王世红, 汪军成, 等. CoCl 2 对NaCl胁迫下大麦生长及幼苗生理指标的影响. 草业学报, 2014, 23(3): 160-166.