Effects of active allelochemicals from feral Gentiana straminea on the seed germination and seedling physiological properties of forages

doi:10.11686/cyxb2016181

Abstract

Abstract: Gentiopicroside and flavonoids were extracted from feral Gentiana straminea using ultrasonic extraction methods and then the extracts were applied at different concentrations to three forages, Medicago sativa, Trifolium pratense and T. repens. The forages’ seed germination and seedling physiological properties (seedling height, root length, fresh weight and the activities of SOD, POD and CAT) were measured to analyze the allelopathy and antioxidation effects of feral G. straminea. In general, the gentiopicroside and flavonoid extracts showed allelopathic inhibitory effects on seed germination and seedling growth in the selected forages. The exception was gentiopicroside at 2.52 mg/mL, which increased seedling height in T. repens. The effects of gentiopicroside and flavonoid extracts on the activity of anti-oxidative enzymes varied with extract concentration. Specifically, both POD and CAT activities in seedlings of the three forages peaked when the concentrations of gentiopicroside and flavonoid extracts were 6.30 and 1.95 mg/mL respectively. When the concentrations of gentiopicroside and flavonoid extracts were 12.60 and 3.90 mg/mL respectively, SOD activity in M. sativa seedlings reached their maximums (22.971 and 25.013 U/g respectively), while in T. pretense and T. repens these activities peaked when the concentrations of gentiopicroside and flavonoid extracts were 6.30 and 1.95 mg/mL respectively. In conclusion, the sampled plants differ in their tolerance to allelopathic stress and M. sativa shows a greater tolerance than either T. pretense and T. repens.

DING Chun-Fa, WEI Xiao-Hong, WANG Fang-Lin. Effects of active allelochemicals from feral Gentiana straminea on the seed germination and seedling physiological properties of forages[J]. Acta Prataculturae Sinica, 2017, 26(4): 150-161.

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