Abstract: In this study, the effects of NaCl at different concentrations (0, 50, 100 and 150 mmol/L) on the growth and osmoregulatory substance accumulation in 60-day-old sugar beet (Beta vulgaris) plants were investigated in pot experiments. The addition of 50, 100, and 150 mmol/L NaCl promoted the growth of sugar beet plants and maintained water conditions well. Compared with the control (0 mmol/L), various concentrations of NaCl significantly increased the fresh weights and dry weights of the leaf blade, leaf petiole, and storage root of B. vulgaris plants (P<0.05). Compared with the control, the high-salt treatment (150 mmol/L) resulted in marked increases in Na⁺ concentrations in the leaf blade and leaf petiole (4.4- and 4.9-fold, respectively; P<0.05), and decreased the relative distribution of Na⁺ in storage roots and lateral roots (by 44% and 53%, respectively; P<0.05). The high-salt treatment also reduced the K⁺ concentrations in the leaf blade and lateral root by 39% and 55%, respectively (P<0.05), and increased the relative distribution of K⁺ in the leaf petiole and storage root by 35% and 80%, respectively (P<0.05). The salt treatments reduced sucrose contents by 44%-50% (P<0.05) and fructose contents in the storage root by 31%-36% (P<0.05), whereas glucose contents were unaffected. The high-salt treatment increased the proline concentration in the storage root by 93%, compared with the control (P<0.05). These results suggested that sugar beet plants can adapt to saline conditions by accumulating the large quantities of Na⁺ in the leaf blade and leaf petiole, by maintaining K⁺ homeostasis, and by enhancing the accumulation of proline in storage roots.