Biological characteristics of Lubao No.1 biological control agent (Colletotrichum gloeosporioides) and construction of a T-DNA insertional mutant library

doi:10.11686/cyxb2016238

Abstract

Abstract: The application of Lubao No.1 microbial agent has been limited because of its degradation, although the strain shows high bio-herbicidal activity against Cuscuta chinensis. The results of an investigation of the biological characteristics of Lubao No.1 showed that the optimal mycelium growth medium was PDA medium. The optimal culture temperature is 25-28 ℃, and optimal initial pH of culture media was 5-8. Construction of a T-DNA insertional mutant library of Lubao No.1 using an Agrobacterium mediated transformation method, produced about 2300 positive transformants. Randomly selected transformants were all resistant to hygromycin B. A hygromycin gene fragment was amplified by PCR from all 20 tested mutants, indicating that the resistance of the transformants to hygromycin B was stable and was caused by the inserted T-DNA. This study laid the foundation for obtaining further applications of a genetically modified Colletotrichum strains with stable pathogenicity.

LI Jian, LI Mei, GAO Xing-Xiang, FANG Feng, DONG Lian-Hong. Biological characteristics of Lubao No.1 biological control agent (Colletotrichum gloeosporioides) and construction of a T-DNA insertional mutant library[J]. Acta Prataculturae Sinica, 2017, 26(1): 142-148.

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