Identification of plant growth promoting rhizobacteria Astragalus membranaceus and their effectives

doi:10.11686/cyxb2016263

Abstract

Abstract: In order to obtain and study the performance of plant growth promoting rhizobacteria (PGPR) in Astragalus membranaceus, the root nodule, root morphology and rhizosphere soil of A. membranaceus were collected. Strains of rhizobium and phosphate solubilizing bacteria were isolated and assessed for the potentially useful characteristics of high nitrogenase activity in the rhizobium and high phosphate solubilisation and ability to secrete 3-indoleacetic acid (IAA) in the phosphate solubilizing bacteria. Potential PGPR strains were then identified using physiological and biochemical characteristics and 16S rDNA sequence analysis. Results showed there are large amounts of phosphorus-dissolving bacteria in the rhizospheres of A. membranaceus. The quantitative distribution of bacteria and PGPR shows a strong rhizosphere effect, with rhizosplan or surface of roots (RP)>soil adhering to roots (RS)>soil away from roots (NRS)>histoplan or interior of roots (HP). We have isolated 76 PGPR strains, composed of 1 rhizobium, 42 inorganic phosphate solubilizing and 33 organic phosphate solubilizing bacteria strains. There are 7 phosphate solubilizing strains with the ability to secrete IAA. A further 8 potential PGPR strains were identified (1 rhizobium and 7 phosphate solubilizing). 3 phosphate solubilizing strains were identified as Pseudomonas sp., 3 phosphate solubilizing strains as Bacillus sp., 1 phosphate solubilizing strain as Klebsiella oxytoca, 1 rhizobium strain as Rhizobium sp. This study has identified potential PGPR for the development of microbial fertilizers for A. membranaceus.

MA Cong-Yu, ZHANG Ying, MA Wen-Bin, LI Jian-Hong, YAO Tuo. Identification of plant growth promoting rhizobacteria Astragalus membranaceus and their effectives[J]. Acta Prataculturae Sinica, 2017, 26(1): 149-159.

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