Drip irrigation of arbuscular mycorrhizal fungal spores and their growth-promoting effects on alfalfa

doi:10.11686/cyxb2016453

Abstract

Abstract: We drew an isoline map of soil moisture content, soil salt content, and spores of arbuscular mycorrhizal fungi (AMF) on alfalfa using Sufer software to analyze the AMF migration state before and after regular drip irrigation. Alfalfa was drip-irrigated at different rates with solutions containing AMF spores. The mycorrhizal infection rate, number of nodules, biomass, and sporulation performance were measured after 50 days of irrigation to evaluate the effects of supplying AMF by this method. The results showed that the number of AMF spores in aqueous solution decreased over time, the soil moisture content was low in upper soil layers and high in deeper soil layers before drip irrigation, and the soil conductivity distribution was uniform. The AMF spores were mainly concentrated in the 10-25 cm soil layer after drip irrigation. The drip water was mostly concentrated within 30 cm of the dripper, and the soil water in this region was higher after drip irrigation than before. Salts were also distributed by the dripper, and concentrated in soil at a distance of 30-45 cm from the dripper. The AMF spores were mainly concentrated within 0-25 cm distance of the dripper, and their abundance decreased with increasing distance from the dripper. Alfalfa growth was affected by drip irrigation of AMF fungal spores and the distance from the dripper. The aboveground biomass and height of alfalfa were significantly higher for plants close to the dripper than for plants more distant from the dripper (P<0.05). The aboveground and belowground biomass of alfalfa were higher for plants irrigated with Gi AMF spore solution than for those in the control (P<0.05). The underground biomass was significantly higher for alfalfa plants irrigated with Gm, Gi, and Ge AMF spore solutions than for those in the control (P<0.05). There was no significant difference in underground biomass of alfalfa between plants irrigated with Gv AMF spore solution and those in the control. The number of alfalfa branches was significantly higher for plants irrigated with Gi AMF spores than for plants in the control (P<0.05). The root nodule number, spore number, and infection rate of alfalfa were significantly higher for plants irrigated with Gi and Ge AMF spore solutions (P<0.05) than for those in the control. The distance from the dripper significantly affected underground biomass, plant height. and number of spores (P<0.05). The interaction between distance from the dripper and AMF strain did not have significant effects for most tested indices, except for infection rate, nodule number, and root length. These results showed that drip irrigation of a solution containing Gi AM fungal spores is beneficial for alfalfa.

JING Peng-Cheng, LV Yan-Ping, WANG Shu-Lin, CHEN Yi-Shi, LU Wei-Hua. Drip irrigation of arbuscular mycorrhizal fungal spores and their growth-promoting effects on alfalfa[J]. Acta Prataculturae Sinica, 2017, 26(9): 121-131.

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