Effect of arbuscular mycorrhizal fungi on growth and physiological characteristics of Lilium brownii

doi:10.11686/cyxb2017001

Abstract

Abstract: Lilium brownii bulbs under cool storage were used to test the effect of inoculated arbuscular mycorrhizal (AM) fungi (Glomus mosseae, G. versiforme and a mix of the two) on bulb growth, physiological and biochemical characteristics in a pot experiment. The results showed that the individual and mixed AM treatments promoted L. brownii growth. Plant height, stem diameter, dry weight above and below ground and leaf surface area increased significantly compared with the non-inoculated control. Of all the inoculation treatments, the mixed fungi had the highest AM colonization. Compared with the control, inoculation with mixed AM significantly improved L. brownii leaf chlorophyll content, superoxide dismutase, peroxidase and catalase content. The inoculation of lily malondialdehyde content was 30% lower than that in the control, while soluble protein and soluble sugar content increased by 25% and 46% respectively, indicating that AM fungi can reduce the damage of membrane lipid oxidation, decrease plant RNA degradation enzymes in the plant, enhance the capacity of the non-enzymatic defense system and increase water retention in plant cells. In addition, inoculation with AM fungi had significant positive effects on leaf net photosynthetic rate, transpiration rate and stomatal conductance.Inoculation with mixed fungi showed significantly higher effects on L. brownii growth and physiological characteristics than non-mixed treatments, while no significant differences were detected between the two strains individually. The study thus shows that G. mosseae and G. versiforme, and especially inoculation with mixes of the two, have the potential to enhance L. brownii growth and flowering.

LIU Zhao-Na, GUO Shao-Xia, LI Wei. Effect of arbuscular mycorrhizal fungi on growth and physiological characteristics of Lilium brownii[J]. Acta Prataculturae Sinica, 2017, 26(11): 85-93.

References

[1] Liu R J, Li X L. Application of AMF[M]. Beijing: Science and Technology Publishing House, 2000.
刘润进, 李晓林. 丛枝菌根及其应用[M]. 北京: 科学技术出版社, 2000.
[2] Song J, Lu S, Chen Z Z, et al . Genetic transformation of Populus trichocarpa genotype nisqually-1: a functional genomic tool for woody plants. Plant Cell Physiology, 2006, 47(11): 1582-1589.
[3] Zeng L, Wang M Y, Li J F. Mechanism and effects of AM fungi on medicinal plants. Journal of Anhui Agricultural Science, 2014, 42(14): 4231-4233.
曾理, 王明元, 李建福. AM真菌对药用植物的影响及其作用机理. 安徽农业科学, 2014, 42(14): 4231-4233.
[4] Jiang L, Li Z M, Huang J G, et al . Influences of arbuscular mycorrhizal fungi on growth and selected physiological indices of tobacco seedlings. Plant Nutrition and Fertilizer Science, 2008, 14(1): 156-161.
江龙, 李竹玫, 黄建国, 等. AM真菌对烟苗生长及某些生理指标的影响. 植物营养与肥料学报, 2008, 14(1): 156-161.
[5] Wu Q S, Zou Y N. Influence of AM fungi on nutrient uptake in leaves of trifoliate orange seedlings subjected to water stress. Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(1): 74-78.
吴强盛, 邹英宁. 水分胁迫下AM真菌对枳实生苗叶片矿质营养吸收的影响. 西北植物学报, 2007, 27(1): 74-78.
[6] Yang X H, Li D G, Takaai I. Effect of weed fermentation matters on growth and vesicular-arbuscular mycorrhizal formation of trifoliate orange seedlings. Acta Horticulturae Sinica, 2001, 28(4): 336-338.
杨晓红, 李道高, Takaai I. 杂草发酵物对枳生长和VA菌根形成的影响. 园艺学报, 2001, 28(4): 336-338.
[7] Feng G, Zhang F S, Li X L, et al . The role and regulation of mycorrhizal fungi in agricultural production. Acta Pedologica Sinica, 2010, 47(5): 995-1004.
冯固, 张福锁, 李晓林, 等. 丛枝菌根真菌在农业生产中的作用与调控. 土壤学报, 2010, 47(5): 995-1004.
[8] Yu G B, Yang X H, Zhu J, et al . Influences of arbuscular mycorrhizal fungi on drought tolerance of pomelo longevity. Southern Agriculture, 2007, 1(1): 24-26.
于桂宝, 杨晓红, 朱钧, 等. AM真菌对长寿沙田柚耐旱性的影响. 南方农业, 2007, 1(1): 24-26.
[9] Qin H B, He C X, Zhang Z B, et al . Effects of arbuscular mycorrhizas fungi on the growth yield and quality of cucumber in greenhouse. Chinese Vegetables, 2008, 21(6): 10-13.
秦海滨, 贺超兴, 张志斌, 等. 丛枝菌根真菌对温室黄瓜生长及产量品质的影响. 中国瓜菜, 2008, 21(6): 10-13.
[10] Li M, Liu R J, Li X L. Influences of arbuscular mycorrhizal fungi on growth and fusarium-wilt disease of watermelon in field. Acta Phytopathologica Sinica, 2004, 34(5): 472-473.
李敏, 刘润进, 李晓林. 大田条件下丛枝菌根真菌对西瓜生长和枯萎病的影响. 植物病理学报, 2004, 34(5): 472-473.
[11] Ren Z, Han L, Zhang Y F, et al . Influences of arbuscular mycorrhizal fungi on the physiological of maize. Jiangsu Agricultural Sciences, 2015, 43(5): 63-66.
任禛, 韩丽, 张永福, 等. 不同丛枝菌根真菌对玉米生长生理的影响. 江苏农业科学, 2015, 43(5): 63-66.
[12] Zhen L N, Zhou H, Yu K M, et al . Effect of AM fungi on the growth relationship between adult plant and seedling under different phosphorus supply levels. Acta Agrestia Sinica, 2015, 23(4): 823-829.
甄莉娜, 周禾, 于肯明, 等. 丛枝菌根真菌与磷添加对成株与幼苗间生长关系的影响. 草地学报, 2015, 23(4): 823-829.
[13] Fan J H. Ecological and Physiological Research on VA Mycorrhizas of Amur Cork-tree[D]. Harbin: Northeast Forestry University, 2006.
范继红. 黄檗丛枝菌根生理生态学研究[D]. 哈尔滨: 东北林业大学, 2006.
[14] Li W, Zhang C P, Wei R P. Relationship of under-story vegetation diversity with stand age and soil factor of eucalypt plantations in central-western Guangdong. Acta Ecologica Sinica, 2014, 34(17): 4957-4965.
李伟, 张翠萍, 魏润鹏. 广东中西部桉树人工林植物多样性与林龄和土壤因子的关系. 生态学报, 2014, 34(17): 4957-4965.
[15] Liu R J, Luo X S. A new method to quantify the inoculum potential of arbuscular mycorrhizal fungi. New Phytologist, 1994, 128(1): 89-92.
[16] Liu R J, Chen Y L. Mycorrhizology[M]. Beijing: Science and Technology Publishing House, 2007: 376-388.
刘润进, 陈应龙. 菌根学[M]. 北京: 科学出版社, 2007: 376-388.
[17] Wang X K. Principles and Techniques of Plant Physiological Biochemical Experiment[M]. Beijing: Higher Education Press, 2006.
王学奎. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2006.
[18] Zhao H B, Zhang Z B, Xu P. Water use efficiency and chlorophyll content of wheat seedling under the inhibition of HgCl 2 . Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(12): 2478-2483.
赵鸿彬, 张正斌, 徐萍. HgCl 2 胁迫对小麦幼苗水分利用效率和叶绿素含量的影响. 西北植物学报, 2007, 27(12): 2478-2483.
[19] Li H S. Principles and Techniques of Plant Physiological Experiment[M]. Beijing: Higher Education Press, 2002: 234-235.
李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2002: 234-235.
[20] Li R L. Biochemistry Experiment[M]. Wuhan: Wuhan University Press, 1998.
李如亮. 生物化学实验[M]. 武汉: 武汉大学出版社, 1998.
[21] Zai X M, Zhang H S, Wang H. Effect of AM fungus and phosphate-solubilizing on the rooting growing of Prunus maritima cuttings in a low phosphorus soil. Northern Horticulture, 2014, (14): 1-5.
宰学明, 张焕仕, 王欢. AM真菌和解磷真菌对低磷土壤滨梅插条生根和生长的影响. 北方园艺, 2014, (14): 1-5.
[22] Yan Z R, Zhang Z L, Xue Y X, et al . Effects of abuscular mycorrhiza fungi on the growth and development of cherry seedling. Guangdong Agricultural Sciences, 2013, 15(3): 47-81.
闫昭如, 张忠兰, 薛元霞, 等. 菌根真菌对樱桃幼苗生长发育的影响. 广东农业科学, 2013, 15(3): 47-81.
[23] Wang Z L, Liu H, Xue Y X, et al . Effect of VA fungi on the growth of apple seedling. Guangdong Agricultural Sciences, 2013, 15(3): 38-40.
王志莉, 刘辉, 薛元霞, 等. VA 菌根真菌对苹果幼苗生长发育的影响. 广东农业科学, 2013, 15(3): 38-40.
[24] Jakobsen I, Rosendahl L. Carbon flow into soil and external hyphae from roots of mycorrhizal cucumber plants. New Phytologist, 1990, 115: 77-83.
[25] Johnson N C, Copeland P J, Crookston R K, et al . Mycorrhizae: possible explanation for yield decline with continuous corn and soybean. Agronomy Journal, 1992, 84: 387-398.
[26] Chen W X. Soil and Environmental Microbiology[M]. Beijing: Beijing Agricultural University Press, 1990: 217-226.
陈文新. 土壤与环境微生物学[M]. 北京: 北京农业大学出版社, 1990: 217-226.
[27] Peng S, Eissenstat D M, Graham J H, et al . Growth depression in mycorrhizal citrus at high phosphorus supply: analysis of carbon costs. Plant Physiology, 1993, 101: 1063-1071.
[28] Zhao Y P, Zhu Y, Qin X J, et al . Influences of arbuscular mycorrhizal fungi on the physiological properties of salvia seedlings. Anhui Agricultural Science Bulletin, 2016, 22(23): 41-42.
赵永平, 朱亚, 秦晓洁, 等. 丛枝菌根真菌对丹参幼苗生理特性的影响. 安徽农学通报, 2016, 22(23): 41-42.
[29] Guo S X, Chen D M, Liu R J. Effects of arbuscular mycorrhizal fungi on antioxidant enzyme activity in peony seedlings under salt stress. Acta Horticulturae Sinica, 2010, 37(11): 1796-1802.
郭绍霞, 陈丹明, 刘润进. 盐水胁迫下接种 AM 真菌对牡丹幼苗抗氧化酶活性的影响. 园艺学报, 2010, 37(11): 1796-1802.
[30] Sun L Y, Xu M, Liu N, et al . Effects of AM fungi on the growth of Tulipa heterophylla . Journal of Qingdao Agricultural University (Natural Science Edition), 2014, 31(4): 242-245.
孙龙燕, 徐萌, 刘宁, 等. AM 真菌对郁金香生长的影响. 青岛农业大学学报(自然科学版), 2014, 31(4): 242-245.
[31] Abu-Zeyad R, Khan A G, Khoo C. Occurrence of arbuscular mycorrhiza in Castanospermum australe A. Cunn. & C. Fraser and effects on growth and production of castanospermine. Mycorrhiza, 1999, 9(2): 111-117.
[32] Ahiabor B D, Hirata H. Characteristic responses of three tropical legumes to the inoculation of two species of VAM fungi in andosol soils with different fertilizers. Mycorrhiza, 1994, 5(1): 63-70.
[33] Han T T, Wang W H, Guo S X. Effect of arbuscular mycorrhizal fungi on photosynthetic characteristics of Coleus blumei . Journal of Qingdao Agricultural University (Natural Science Edition), 2011, 28(1): 9-12.
韩婷婷, 王维华, 郭绍霞. AM真菌对彩叶草光合作用的影响. 青岛农业大学学报(自然科学版), 2011, 28(1): 9-12.
[34] Ma Y B, Li W, Xu M, et al . Effect of arbuscular mycorrhizal fungi on growth and photosynthesis of lily under salt stress. Journal of Qingdao Agricultural University (Natural Science Edition), 2014, 31(3): 157-161.
马亚斌, 李伟, 徐萌, 等. AM 真菌对盐胁迫下百合生长和光合作用的影响. 青岛农业大学学报(自然科学版), 2014, 31(3): 157-161.
[35] Sheng M, Tang M, Chen H, et al . Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress. Mycorrhiza, 2008, 18(6): 287-296.