Cloning and functional analysis of halophyte Halogeton glomeratus HgNHX1 promoter

doi:10.11686/cyxb2017018

Abstract

Abstract: In order to test the function of pHgNHX1, the promoter sequence of the HgNHX1 gene from Halogeton glomeratus was cloned and a 1523 bp fragment flanking 5'-upstream of HgNHX1 was isolated and named pHgNHX1. The software PlantCARE and PLACE were used to predict and analyze the elements of pHgNHX1. The results showed that besides the TATA-box and CAAT-box, a number of potential cis-elements and transcription binding motifs related to stress responses were found, including salt, dehydration, cold and wound responsive elements. Other potential cis-elements responsive to phytohormones, including auxin, abscisic acid, gibberellin and ethylene were also found in the sequence. Sequence analysis indicated that it had the general characteristics of typical promoter. In order to evaluate the activity of pHgNHX1, we constructed pBI-pHgNHX1 expression vector and introduced it into tobacco and Arabidopsis using agrobacterium mediated transformation. The expression pattern was monitored using GUS histochemical staining. Results showed that GUS activity driven by the pHgNHX1 was detected in almost all vegetative and reproductive tissues, indicating that pHgNHX1 has a constitutive promoter activity.

ZOU Lan, YANG Ke, XU Xian-Liang, WANG Jun-Cheng, REN Pan-Rong, YAO Li-Rong, MENG Ya-Xiong, LI Bao-Chun, MA Xiao-Le, WANG Hua-Jun. Cloning and functional analysis of halophyte Halogeton glomeratus HgNHX1 promoter[J]. Acta Prataculturae Sinica, 2017, 26(11): 57-68.

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