Effect of manganese stress on seed germination, seedling physiological and biochemical characteristics of Xanthium sibiricum

doi:10.11686/cyxb2017033

Abstract

Abstract: To investigate the physiological and biochemical adaption mechanisms of Xanthium sibiricum to manganese (Mn) stress, Xanthium sibiricum seeds were treated with different concentrations of Mn (0, 1000, 5000, 10000, 15000 and 20000 μmol/L) and subsequently germinated on filter paper. Seed germination, seedling growth, physiological and biochemical characteristics of seedlings were examined. 1) Compared with the control, germination rate, bud length and root vigor increased significantly at 1000-5000 μmol/L Mn. Germination potential, germination index, vigor index, root length, fresh weight, dry weight and root-shoot ratio significantly decreased with Mn concentrations over 5000 μmol/L. 2) Soluble sugar, soluble protein, proline and superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and catalase (CAT) initially increased and then decreased with the rising Mn concentration. 3) Reactive oxygen species (ROS) and malondialdehyde (MDA) in seedlings increased significantly with the increase in Mn concentration. The O2· - (superoxide anion radical), OH^· (hydroxyl radical), H₂O₂ and MDA content increased by 154.69%, 47.29%, 100.09% and 200.96% at the highest Mn concentration, respectively. It showed that X. sibiricum has strong Mn tolerance and adaptability, and is potentially utilizable for phytoremediation.

PAN Gao, ZHANG He-Ping, LIU Peng, LIU Wen-Sheng. Effect of manganese stress on seed germination, seedling physiological and biochemical characteristics of Xanthium sibiricum[J]. Acta Prataculturae Sinica, 2017, 26(11): 157-166.

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