Construction of RNAi expression vector of CYP86A gene in halophyte Puccinellia tenuiflora

doi:10.11686/cyxb2017076

Abstract

Abstract: The salt-excluding halophyte Puccinellia tenuiflora exhibits a highly suberized endodermis in the root, which may contribute to its salt tolerance. In this study, the RNAi target fragment of CYP86A, a key gene in suberin biosynthesis, was amplified by RT-PCR from roots of P. tenuiflora seedlings under salt stress. Then based on the intermediate vector pHANNIBAL and the plant expression vector pART27, the RNAi plant expression vector pARC, which contained inverted repeats of PtCYP86A and was driven by the 35S promoter, was constructed successfully by restriction enzyme digestion, ligation and In-Fusion cloning. The work might be a good basis for further investigating the roles of roots suberization in Na⁺ exclusion in P. tenuiflora by RNAi technique.

WANG Pei, CUI Yan-Nong, GAO Li, WANG Suo-Min. Construction of RNAi expression vector of CYP86A gene in halophyte Puccinellia tenuiflora[J]. Acta Prataculturae Sinica, 2017, 26(6): 105-110.

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