Fitness of resistant backcross generation (BC3F2-3) between glufosinate-resistant transgenic oilseed rape and wild Brassica juncea

doi:10.11686/cyxb2017129

Abstract

Abstract: One of the concerns about releasing transgenic herbicide-resistant oilseed rape (Brassica napus, AACC=38) is that herbicide-resistant (HR) transgenes from transgenic oilseed rape may escape to wild Brassica juncea. If this happens, wild B. juncea with the HR trait might pose new problems for weed control. Therefore, it is necessary to evaluate gene flow from HR transgenic oilseed rape to wild B. juncea before it is released. The fitness components and composite fitness of BC3mF2, BC3pF2, BC3mF3 and BC3pF3 (m=backcross progeny obtained with wild B. juncea as maternal plants; p=backcross progeny obtained with wild B. juncea as paternal plants.) between glufosinate-resistant transgenic oilseed rape and wild B. juncea under different densities and different planting proportions were measured in the field. In pure plots, the composite fitness of BC3F2 and BC3F3 were not different to wild B. juncea either at low density (15 plants/plot), or high density (30 plants/plot). In mixed plots, at low density under 4:1, 3:2 proportions, the composite fitness of the backcross generations was lower than that of wild B. juncea except for BC3mF3, which was similar to wild B. juncea. Under 1:1 proportion, the composite fitness of the backcross generations was lower than that of wild B. juncea except for BC3mF2 and BC3mF3, which were similar to wild B. juncea. At high density, the composite fitness of all backcross generations was lower than that of wild B. juncea irrespective of proportions. There was no correlation between fitness components of BC3mF3 and planting density. BC3F2 and BC3F3 between glufosinate-resistant transgenic oilseed rape and wild B. juncea have the ability to establish in field, particularly BC3mF3. Therefore, hybridization between transgenic oilseed rape and wild B. juncea, including backcrosses between wild B. juncea and F₁ or subsequent generations, should be prevented.

WANG Xiao-Lei, WANG Jian, ZHANG Qing-Ling, YAN Jing, QIANG Sheng, SONG Xiao-Ling. Fitness of resistant backcross generation (BC3F2-3) between glufosinate-resistant transgenic oilseed rape and wild Brassica juncea[J]. Acta Prataculturae Sinica, 2017, 26(12): 138-151.

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