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Acta Prataculturae Sinica ›› 2017, Vol. 26 ›› Issue (11): 77-84.DOI: 10.11686/cyxb2017145

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Effect of 5 strains of Lactic acid bacteria with antibacterial activity on the corn silage quality

LEI Zhao-Min1, WANG Jian-Fu1, WU Jian-Ping1, HE Yi-Qun2, WU Run2, JIANG Hui1, WAN Xue-Rui2,*   

  1. 1.College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2.College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2017-03-28 Revised:2017-05-31 Online:2017-11-20 Published:2017-11-20

Abstract: To investigate the effect of lactic acid bacteria (LAB) with antibacterial activity on the quality of corn silage during ensiling, and screen LAB inoculant that could improve the quality of silage, 5 strains of LAB (B1-7, B2-3, B3-1, B5-2, E2-3) isolated from corn silage across Gansu province were added to make corn silage, the contents of dry matter (DM), crude protein (CP), water soluble carbohydrates (WSC), neutral detergent fiber (NDF), acid detergent fiber (ADF), ammonia nitrogen (NH3-N), lactic acid (LA), acetic acid (AA), propionic acid (PA) and butyric acid (BA) were analyzed. Results showed that for silage at 30 days, the content of DM, CP, ADF and LA was significantly increased in 5 LAB strains treatment groups compared with the control group, and the content of NH3-N and WSC was decreased. The NDF content in silage inoculated with the B3-1 LAB strain was significantly higher than the control (P<0.05), with the other 4 LAB strains being intermediate, while the content of AA in B3-1 and B5-2 treated silages was significantly higher than the control but not the other 3 treatment groups. Along with the fermentation time the content of dry matter, crude protein, water soluble carbohydrates in silages of each group was decreased and the content of ADF, NDF and NH3-N increased. Overall, the silages inoculated with the B3-1 strain showed best fermentation quality and nutritive value characteristics. These results indicated that all added LAB strains improved the quality of silage, with Lactobacillus plantarum B3-1 being the most effective.