Cloning, subcellular localization and expression analysis of a novel phytoene synthase gene, ZmPSY, in Zoysia matrella

doi:10.11686/cyxb2017176

Abstract

Abstract: The formation of phytoene by phytoene synthase is a key limiting step in the carotenoid biosynthesis pathway. In this study a novel gene, ZmPSY (GenBank Accession number: KY264128), was cloned from Zoysia matrella. Subcellular localization results proved that ZmPSY was localized in the chloroplasts. Expression analysis showed that ZmPSY had a higher expression level in leaves compared to roots and stems. The expression of ZmPSY in leaves also varied at different development stages, with the greatest expression seen in young leaves. qRT-PCR determination revealed that ZmPSY could be induced by exogenous abscisic acid (ABA), but suppressed by salicylic acid (SA) and methyl jasmonate (MeJA). This study paved the way for further discovery of the functional roles of ZmPSY.

DONG Di, TENG Ke, YU An-Dong, TAN Peng-Hui, LIANG Xiao-Hong, HAN Lie-Bao. Cloning, subcellular localization and expression analysis of a novel phytoene synthase gene, ZmPSY, in Zoysia matrella[J]. Acta Prataculturae Sinica, 2017, 26(11): 69-76.

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