Cloning and expression analysis of LpAGD14 from Lilium pumilum

doi:10.11686/cyxb2017317

Abstract

Abstract: A gene encoding an ArfGAP protein was cloned from the cDNA library of Lilium pumilum. Its ORF sequence encoded a 640-amino acid polypeptide with a typical C4 ArfGAP conserved domain. The relative molecular mass of the putative ArfGAP protein was 69.43 kDa, the theoretical isoelectric point was 8.95, and it was predicted to be a hydrophilic protein with no transmembrane structure. At the amino acid sequence level, the L. pumilum ArfGAP showed 59% homology to ArfGAP-domain proteins (AGDs) in Elaeis guineensis, and more than 50% homology to AGDs in Musa acuminata subsp., Phoenix dactylifera, and Ananas comosus. Therefore, the gene was named LpAGD14. Transcript analysis by qRT-PCR showed that the transcript levels of LpAGD14 increased to reach the highest value after dormancy release. Subcellular localization assays showed that LpAGD14 localized to the cell membrane. Ultrastructural observations showed that the layers of the Golgi body increased with prolonged cold storage. These results indicated that LpAGD14 is involved in the dormancy release of L. pumilum and plays a role in vesicle transport. This study lays the foundation for further research on the function and regulation of this gene and its product.

WANG Wang, TIAN Zhong-ping, SU Xiao-xia, YANG Liu-hui, ZHOU Yun-wei. Cloning and expression analysis of LpAGD14 from Lilium pumilum[J]. Acta Prataculturae Sinica, 2018, 27(2): 117-123.

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