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Acta Prataculturae Sinica ›› 2018, Vol. 27 ›› Issue (3): 135-143.DOI: 10.11686/cyxb2017321

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Response of microbial C, N and respiration characteristics to sowing rates in alfalfa cultivation grasslands in Hulunber, Inner Mongolia

WANG Di1, PANG Huan-cheng1, LI Da1, CHEN Jin-qiang1, XIN Xiao-ping1, XU Li-jun1,*, TANG Xue-juan1, GUO Ming-ying2, ZHU Shu-sheng3   

  1. 1.Institute of Agricultural Resources and Regional Planning of Chinese Academy of Agricultural Sciences, Hulunber Grassland Ecosystem Observation and Research Station, Beijing 100081, China;
    2.Hulunber Grassland Ecosystem Observation and Research Station, Hailar 021200, China;
    3.Yakeshi Grassland Ecosystem Observation and Research Station, Yakeshi 022150, China;
  • Received:2017-08-10 Revised:2017-10-26 Online:2018-03-20 Published:2018-03-20

Abstract: This study investigated changes in microbial biomass carbon (MBC), microbial biomass nitrogen (MBN) and the respiration characteristics of alfalfa under 5 different sowing rates (6, 9, 12, 15 and 18 kg·ha-1). The study was undertaken from July to September 2015 in Hulunber, Inner Mongolia. The results showed that aboveground and underground biomass reached the maximum when the sowing amount was 12 kg·ha-1. The highest MBC value appeared at 18 kg·ha-1 sowing quantity among all soil layers and months. As a whole, the value of MBC presented higher in July than September, with this difference more significant at the 0-10 cm than 10-20 cm soil layers. MBN values also presented higher in July than September. Changes in soil respiration entropy (qCO2) were coincident with microbial respiration, with the lowest qCO2 levels appearing in the 12 kg·ha-1 sowing treatment. There were no significant differences in microbial respiration and qCO2 between the 18 kg·ha-1 and 12 kg·ha-1 treatments in July and September. We found that MBC, microbial respiration and qCO2 varied according to the sowing rate of alfalfa. To summarize, taking into consideration such factors as aboveground biomass, microbial respiration and qCO2, our study suggests that the best sowing quantity for alfalfa cultivation in Hulunber is 18 kg·ha-1.