Analysis of stress-resistance of CgDREB22 from Chrysanthemum×grandiflora in tabacco

doi:10.11686/cyxb2019552

Abstract

Abstract: DREB transcription factor is a subfamily of the AP2/ERF transcription factors, which is involved in the molecular response mechanism of plants to enviromental stresses such as drought, salinity and low temperature. The CgDREB22 gene from Chrysanthemum×grandiflora was incorporated into tobacco using an agrobacterium-mediated method. Seeds and seedlings of the T₁ generation transgenic tobacco were treated with 150 mmol·L^-1 NaCl, 150 mmol·L^-1 mannitol, and low temperature (4 ℃). It was found that under NaCl exposure, osmotically simulated drought stress, and low temperature stress, the fresh weight and root length of transgenic tobacco were lower than in untransformed comparisons. Under NaCl exposure and osmotically simulated drought stress, the resistance mechanism of transgenic tobacco was destroyed,and the proline content and antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) of transgenic tobacco were significantly lower than the untransformed comparison plants. In summary, the CgDREB22 plays a negative role in regulating environmental stresses in plants.

Key words: Chrysanthemum×grandiflora, CgDREB22, tobacco, enviromental stresses

HUANG Qin-mei, YANG Yi-ru, CHEN Li-fei, YIN Hang, LIU He, LIU Ying-jie, ZHOU Yun-wei, HE Miao. Analysis of stress-resistance of CgDREB22 from Chrysanthemum×grandiflora in tabacco[J]. Acta Prataculturae Sinica, 2020, 29(10): 109-118.

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