Effects of nitrogen application rate on chlorophyll fluorescence characteristics and dry matter accumulation in switchgrass (Panicum virgatum) leaves

doi:10.11686/cyxb2020007

Abstract

Abstract: The effect of nitrogen fertilizer application rate on chlorophyll fluorescence characteristics and dry matter accumulation of switchgrass (Panicum virgatum) is of great significance for the improvement of light use efficiency and biomass yield of switchgrass in saline-alkali land. In this research, the patterns of variation in chlorophyll fluorescence parameters and dry matter accumulation in switchgrass were studied in field conditions with a range of nitrogen application treatments: no nitrogen (0 kg·ha^-1, N₀), low nitrogen application (60 kg·ha^-1, N₆₀), medium nitrogen application (120 kg·ha^-1, N₁₂₀) and high nitrogen application (240 kg·ha^-1, N₂₄₀), and the comprehensive effect of nitrogen application on chlorophyll fluorescence and dry matter accumulation in switchgrass was evaluated using a multivariate Grey relational analysis procedure. It was found that: under N₆₀, N₁₂₀ and N₂₄₀ treatments, the PSⅡ maximum photochemical efficiency (F_v/F_m), PSⅡ actual photochemical quantum efficiency (Φ_PSⅡ), potential activity (F_v/F_o), photochemical quenching (qP), non photochemical quenching (NPQ) and biomass yield were significantly higher, and the heat dissipation of quantum ratio (F_o/F_m) was significantly lower than for the N₀ treatment during the flowering and seed maturation stages of switchgrass development. During the flowering period of switchgrass, the PSⅡ potential activity (F_v/F_o) was initially increased and subsequently decreased across the range of nitrogen application rates, with a maximum observed value of 3.13 at N₁₂₀ (increased 16.26% compared with N₀). The effect of nitrogen application rate on dry matter accumulation differed between the crop growth stages. Dry matter accumulation was high during jointing and booting periods, reached its maximum at the seed filling stage, and decreased slightly thereafter. The maximum observed dry matter accumulation (378.13 g·hole^-1) occurred under the N₂₄₀ treatment during the post-flowering stage and was, respectively, 24.33%, 20.09% and 7.24% higher than that of the N₀, N₆₀ and N₁₂₀ treatments. Grey relational analysis showed that the association between the weighted correlation index and the ideal fertilization level was the highest under the N₂₄₀ treatment. In summary, the N₂₄₀ treatment in this study in the Yinbei saline-alkali area of Ningxia optimized the photochemical activity of PSⅡ and dry matter accumulation of switchgrass.

Key words: nitrogen application, switchgrass (Panicum virgatum), chlorophyll fluorescence, grey relational analysis

HE Hai-feng, YAN Cheng-hong, WU Na, LIU Ji-li, CHANG Wen-wen. Effects of nitrogen application rate on chlorophyll fluorescence characteristics and dry matter accumulation in switchgrass (Panicum virgatum) leaves[J]. Acta Prataculturae Sinica, 2020, 29(11): 141-150.

References

[1] Xu S N, Fan L X, He Y, et al. Effects of different nitrogen application rates on the growth and photosynthetic fluorescence characteristics of zoysiagrass. Soil and Fertilizer Science in China, 2013, (5): 46-50.
徐苏男, 范丽霞, 何月, 等. 不同施氮量对结缕草生长及光合荧光特性的影响. 中国土壤与肥料, 2013, (5): 46-50.
[2] Li L L, Fang W P, Xie D Y, et al. Effects of nitrogen application rate on photosynthetic physiological characteristics, yield and quality of hybrid cotton. Plant Nutrition and Fertilizer Science, 2010, 16(5): 1183-1189.
李伶俐, 房卫平, 谢德意, 等. 施氮量对杂交棉光合生理特性及积累量、品质的影响. 植物营养与肥料学报, 2010, 16(5): 1183-1189.
[3] Wu W M, Chen H J, Li J C, et al. Effects of nitrogen application strategies on chlorophyll fluorescence and grain filling characteristics of winter wheat in the booting stage. Acta Agronomica Sinica, 2012, 38(6): 1088-1096.
武文明, 陈洪俭, 李金才, 等. 氮肥运筹对成熟期受渍冬小麦旗叶叶绿素荧光与籽粒灌浆特性的影响. 作物学报, 2012, 38(6): 1088-1096.
[4] Jiao Y J, Yin C Y, Chen K. Effects of soil water and nitrogen supply on photosynthetic characteristics of Jatropha curcas seedlings. Chinese Journal of Plant Ecology, 2011, 35(1): 91-99.
焦玉娟, 尹春英, 陈珂. 土壤水、氮供应对麻疯树幼苗光合特性的影响. 植物生态学报, 2011, 35(1): 91-99.
[5] Jiang H M, Zhang J F, Yang J C, et al. Effects of different nitrogen fertilizer application on tomato yield, quality and soil nitrate nitrogen accumulation in facilities. Journal of Agro-Environment Science, 2010, 29(12): 2338-2345.
姜慧敏, 张建峰, 杨俊诚, 等. 不同氮肥用量对设施番茄积累量、品质和土壤硝态氮累积的影响. 农业环境科学学报, 2010, 29(12): 2338-2345.
[6] Xiang F, Li W, Liu H Y, et al. Effects of nitrogen levels on photosynthesis and chlorophyll fluorescence characteristics of different tea plants. Acta Botanica Boreali-Occidentalia Sinica, 2018, 38(6): 1138-1145.
向芬, 李维, 刘红艳, 等. 氮素水平对不同品种茶树光合及叶绿素荧光特性的影响. 西北植物学报, 2018, 38(6): 1138-1145.
[7] Li Q, Luo Y H, Yu D H, et al. Effects of low nitrogen stress on photosynthesis and chlorophyll fluorescence characteristics of low nitrogen tolerant maize varieties. Plant Nutrition and Fertilizer Science, 2015, 21(5): 1132-1141.
李强, 罗延宏, 余东海, 等. 低氮胁迫对耐低氮玉米品种苗期光合及叶绿素荧光特性的影响. 植物营养与肥料学报, 2015, 21(5): 1132-1141.
[8] Tan X L, Guo T W, Zhang G H, et al. Effects of nitrogen on chlorophyll fluorescence parameters in different leaf positions of wheat. Journal of Triticeae Crops, 2009, 29(3): 437-441.
谭雪莲, 郭天文, 张国宏, 等. 氮素对小麦不同叶位叶片叶绿素荧光参数的调控效应. 麦类作物学报, 2009, 29(3): 437-441.
[9] Hao X Y, Han X, Li P, et al. Effects of elevated atmospheric CO₂ concentration on photosynthesis and chlorophyll fluorescence parameters of green bean leaves. Chinese Journal of Applied Ecology, 2011, 22(10): 2776-2780.
郝兴宇, 韩雪, 李萍, 等. 大气CO₂浓度升高对绿豆叶片光合作用及叶绿素荧光参数的影响. 应用生态学报, 2011, 22(10): 2776-2780.
[10] Ye Z P, Hu W H, Yan X H. Comparison on light-response models of actual photochemical efficiency in photosystem Ⅱ. Chinese Journal of Plant Ecology, 2016, 40(11): 1208-1217.
叶子飘, 胡文海, 闫小红. 光系统Ⅱ实际光化学量子效率对光的响应模型的比较. 植物生态学报, 2016, 40(11): 1208-1217.
[11] Yan Y H, Yang W Y, Zhang X Q, et al. Effects of nitrogen application amount on photosynthetic characteristics, dry matter accumulation and yield after interplanting soybean. Acta Prataculturae Sinica, 2011, 20(3): 233-238.
闫艳红, 杨文钰, 张新全, 等. 施氮量对套作大豆花后光合特性、干物质积累及产量的影响. 草业学报, 2011, 20(3): 233-238.
[12] Liu X J, Liu Y N, Kuai J L, et al. Effects of nitrogen supply levels on alfalfa accumulation and quality. Acta Agrestia Sinica, 2013, 21(4): 702-707.
刘晓静, 刘艳楠, 蒯佳林, 等. 供氮水平对不同紫花苜蓿积累量及品质的影响. 草地学报, 2013, 21(4): 702-707.
[13] Huang J, Gao Z J, Wang S Q, et al. Effects of nitrogen on tillering stage and photosynthetic physiological characteristics of switchgrass. Chinese Journal of Grassland, 2018, 40(6): 10-17.
黄瑾, 高志娟, 王世琪, 等. 氮素对分蘖期干旱及复水柳枝稷光合生理特性的影响. 中国草地学报, 2018, 40(6): 10-17.
[14] Liu J L, Zhu W B, Xie G H, et al. Research progress on energy crops switchgrass. Acta Prataculturae Sinica, 2009, 18(3): 232-240.
刘吉利, 朱万斌, 谢光辉, 等. 能源作物柳枝稷研究进展. 草业学报, 2009, 18(3): 232-240.
[15] Danielle C, Hyemi K, Lauren Q, et al. Switchgrass as a bioenergy crop in the Loess Plateau, China: Potential lignocellulosic feedstock production and environmental conservation. Journal of Integrative Agriculture, 2017, 16(6): 1211-1226.
[16] Chang W W, Liu J L, Wu N, et al. Comparison of photosynthetic characteristics and yield of different switchgrass cultivars in saline-alkali regions in Northwest China. Acta Agriculturae Zhejiangensis, 2019, 31(10): 1647-1654.
常雯雯, 刘吉利, 吴娜, 等. 西北盐碱地区不同柳枝稷品种光合特性与积累量比较. 浙江农业学报, 2019, 31(10): 1647-1654.
[17] Deng J L. Course of grey system theory. Wuhan: Huazhong University of Science and Technology Press, 1990.
邓聚伦. 灰色系统理论教程. 武汉: 华中科技大学出版社, 1990.
[18] Long J R, Ma G H, Wan Y Z, et al. Effects of nitrogen fertilizer level on chlorophyll fluorescence characteristic in flag leaf of super hybrid rice at late growth stage. Chinese Journal of Rice Science, 2011, 25(5): 501-507
龙继锐, 马国辉, 万宜珍, 等. 施氮量对超级杂交中稻生育后期剑叶叶绿素荧光特性的影响. 中国水稻科学, 2011, 25(5): 501-507.
[19] Huo L J, Ding W L, Gao Z J, et al. Chlorophyll fluorescence parameters and response characteristics to water and nitrogen conditions of switchgrass under mixed seeding. Acta Botanica Boreali-Occidentalia Sinica, 2016, 36(4): 757-765.
霍丽娟, 丁文利, 高志娟, 等. 混播下柳枝稷叶绿素荧光参数及对水氮条件的响应特征. 西北植物学报, 2016, 36(4): 757-765.
[20] Sun Y, Wu C S, Gu Y. Effects of nitrogen fertilizer on chlorophyll fluorescence characteristics and yield of corn leaves under membrane drip irrigation in semi-arid area. Journal of Maize Sciences, 2017, 25(1): 133-138, 146.
孙扬, 吴春胜, 谷岩. 氮肥对半干旱区膜下滴灌玉米叶片叶绿素荧光特性及产量的影响. 玉米科学, 2017, 25(1): 133-138, 146.
[21] Bai Z Y, Li C D, Zhao J F, et al. Effects of drought stress on chlorophyll fluorescence parameters and chromosome effects of wheat substitution lines. Scientia Agricultura Sinica, 2011, 44(1): 47-57.
白志英, 李存东, 赵金锋, 等. 干旱胁迫对小麦代换系叶绿素荧光参数的影响及染色体效应初步分析. 中国农业科学, 2011, 44(1): 47-57.
[22] Kouki H. Interspecific difference in the photosynthesis-nitrogen relationship: Patterns, physiological causes, and ecological importance. Journal of Plant Research, 2004, 117: 481-494.
[23] Hong C O, Owens V N, Bransby D, et al. Switchgrass response to nitrogen fertilizer across diverse environments in the USA: A regional feedstock partnership report. Bioenergy Research, 2014, 7(3): 777-788.
[24] Kimura E, Collins H P, Fransen S. Biomass production and nutrient removal by switchgrass under irrigation. Agronomy Journal, 2015, 107(1): 204-210.
[25] Pang L D, Li W J, Zhu J Z. Effects of topdressing nitrogen fertilizer on photosynthetic characteristics and seed yield of Sudan grass. Pratacultural Science, 2014, 31(12): 2286-2292.
庞立东, 李卫军, 朱进忠. 追施氮肥对苏丹草光合特性及种子产量的影响. 草业科学, 2014, 31(12): 2286-2292.
[26] Liu J L, Wu N. Effects of nitrogen application on biomass yield and utilization characteristics of water and fertilizer from switchgrass. Acta Agriculturae Boreali-Occidentalis Sinica, 2014, 23(7): 166-171.
刘吉利, 吴娜. 施氮量对柳枝稷生物质产量和水肥利用特性的影响. 西北农业学报, 2014, 23(7): 166-171.
[27] Tao M, Wang T M, Su D R, et al. Effect of nitrogen fertilizer on the target yield of switchgrass in Kubuqi Sand. Journal of Beijing Forestry University, 2017, 39(7): 87-95.
陶梦, 王铁梅, 苏德荣, 等. 氮肥对库布齐沙地柳枝稷目标产量的影响. 北京林业大学学报, 2017, 39(7): 87-95.
[28] Xu Y X, Ding X Y, Huang X Y, et al. Effects of nitrogen fertilizer level on biological yield and quality of silage maize in mildly saline-alkali land. Heilongjiang Animal Science and Veterinary Medicine, 2017, (10): 181-183.
徐艳霞, 丁昕颖, 黄新育, 等. 轻度盐碱地氮肥水平对青贮玉米生物产量和品质的影响. 黑龙江畜牧兽医, 2017, (10): 181-183.
[29] Zhang Y Q. Study on the adaptability of switchgrass in saline-alkali land in Yinbei area of Ningxia. Yinchuan: Ningxia University, 2018.
张永乾. 宁夏银北地区盐碱地柳枝稷适应性研究. 银川: 宁夏大学, 2018.