Acta Prataculturae Sinica ›› 2022, Vol. 31 ›› Issue (4): 145-154.DOI: 10.11686/cyxb2021021
De-zhi YANG1, Chen WANG2, Ming-jie HOU3, Hu-cheng WANG2()
Received:
2021-01-20
Revised:
2021-03-22
Online:
2022-04-20
Published:
2022-01-25
Contact:
Hu-cheng WANG
De-zhi YANG, Chen WANG, Ming-jie HOU, Hu-cheng WANG. Effects of sweet sorghum silage and whole-plant corn silage on the forestomach microecology of mutton sheep[J]. Acta Prataculturae Sinica, 2022, 31(4): 145-154.
项目Items | 引物序列Primer sequences (5′-3′) | 文献References |
---|---|---|
白色瘤胃球菌 Rumincoccus albus | F: CCCTAAAAGCAGTCTTAGTTCG | [ |
R: CCTCCTTGCGGTTAGAAC | ||
产琥珀酸丝状杆菌 Fibrobacter succinogenes | F: GGTATGGGATGAGCTTGC | [ |
R: GCCTGCCCCTGAACTATC | ||
黄色瘤胃球菌 Rumincoccus flavefaciens | F: TCTGGAAACGGATGGTA | [ |
R: CCTTTAAGACAGGAGTTTACAA | ||
溶纤维丁酸弧菌 Butyrivibrio fibrisolvens | F: GCCTCAGCGTCAGTAATCG | [ |
R: GGAGCGTAGGCGGTTTTAC | ||
短普雷沃氏菌 Prevotella brevis | F: GCGAACTGGTTTCCTTGAGTGTATT | [ |
R: ACCTTCGAGCTTTAGCGTCAGTTAT | ||
栖普雷沃氏菌 Prevotella ruminicola | F: GCGAAAGTCGGATTAATGCTCTATG | [ |
R: CCCATCCTATAGCGGTAAACCTTTG | ||
总菌 Total bacteria | F: CGGCAACGAGCGCAACCC | [ |
R: CCATTGTAGCACGTGTGTAGCC |
Table 1 Forestomach microorganisms RT-PCR primer
项目Items | 引物序列Primer sequences (5′-3′) | 文献References |
---|---|---|
白色瘤胃球菌 Rumincoccus albus | F: CCCTAAAAGCAGTCTTAGTTCG | [ |
R: CCTCCTTGCGGTTAGAAC | ||
产琥珀酸丝状杆菌 Fibrobacter succinogenes | F: GGTATGGGATGAGCTTGC | [ |
R: GCCTGCCCCTGAACTATC | ||
黄色瘤胃球菌 Rumincoccus flavefaciens | F: TCTGGAAACGGATGGTA | [ |
R: CCTTTAAGACAGGAGTTTACAA | ||
溶纤维丁酸弧菌 Butyrivibrio fibrisolvens | F: GCCTCAGCGTCAGTAATCG | [ |
R: GGAGCGTAGGCGGTTTTAC | ||
短普雷沃氏菌 Prevotella brevis | F: GCGAACTGGTTTCCTTGAGTGTATT | [ |
R: ACCTTCGAGCTTTAGCGTCAGTTAT | ||
栖普雷沃氏菌 Prevotella ruminicola | F: GCGAAAGTCGGATTAATGCTCTATG | [ |
R: CCCATCCTATAGCGGTAAACCTTTG | ||
总菌 Total bacteria | F: CGGCAACGAGCGCAACCC | [ |
R: CCATTGTAGCACGTGTGTAGCC |
项目Item | SS | CS |
---|---|---|
干物质采食量Dry matter intake (DMI, kg·d-1) | 0.98±0.05b | 1.10±0.04a |
平均日增重Average daily gain (ADG, kg·d-1) | 0.11±0.03b | 0.16±0.30a |
料重比F/G | 9.11±2.16a | 6.89±1.35b |
Table 2 Effects of sweet sorghum and whole-plant corn silage on the growth performance of mutton sheep
项目Item | SS | CS |
---|---|---|
干物质采食量Dry matter intake (DMI, kg·d-1) | 0.98±0.05b | 1.10±0.04a |
平均日增重Average daily gain (ADG, kg·d-1) | 0.11±0.03b | 0.16±0.30a |
料重比F/G | 9.11±2.16a | 6.89±1.35b |
项目 Item | 瘤胃 Rumen | 网胃 Reticulum | 瓣胃 Omasum | |||
---|---|---|---|---|---|---|
SS | CS | SS | CS | SS | CS | |
pH | 6.39±0.06a | 6.28±0.64a | 6.43±0.65a | 7.13±0.79a | 6.51±0.79a | 6.13±0.52a |
氨氮Ammonia nitrogen (NH3-N, g·dL-1) | 3.23±1.10a | 1.97±0.24a | 2.63±0.26a | 1.61±0.03b | 3.38±0.77a | 3.64±1.39a |
总挥发性脂肪酸Total volatile fatty acids (TVFA, mmol·L-1) | 44.12±4.73a | 40.13±1.60b | 36.73±5.33a | 36.68±4.17a | 36.66±3.23a | 33.37±4.49a |
乙酸Acetic acid (%) | 70.60±10.49a | 73.91±2.85a | 72.85±3.37a | 73.48±0.54a | 78.87±1.41a | 78.95±2.68a |
丙酸Propionic acid (%) | 18.14±6.87a | 16.46±1.82a | 17.49±2.55a | 16.21±0.66a | 14.72±0.87a | 14.87±2.03a |
异丁酸Isobutyric acid (%) | 0.91±0.56a | 0.50±0.13a | 0.53±0.18a | 0.75±0.11a | 0.51±0.17a | 0.70±0.16a |
丁酸Butyric acid (%) | 7.91±2.56a | 7.15±1.83a | 6.10±0.70b | 7.37±0.45a | 2.79±1.10a | 3.16±0.83a |
异戊酸Isovaleric acid (%) | 1.26±0.56a | 1.52±0.09a | 1.20±0.35a | 1.45±0.15a | 1.11±0.50a | 1.60±0.03a |
戊酸Valeric acid (%) | 1.17±0.94a | 0.83±0.21a | 1.83±2.48a | 0.75±0.21a | 2.00±2.79a | 0.72±0.03a |
Table 3 Effects of sweet sorghum and whole-plant corn silage on fermentation parameters of forestomach in mutton sheep
项目 Item | 瘤胃 Rumen | 网胃 Reticulum | 瓣胃 Omasum | |||
---|---|---|---|---|---|---|
SS | CS | SS | CS | SS | CS | |
pH | 6.39±0.06a | 6.28±0.64a | 6.43±0.65a | 7.13±0.79a | 6.51±0.79a | 6.13±0.52a |
氨氮Ammonia nitrogen (NH3-N, g·dL-1) | 3.23±1.10a | 1.97±0.24a | 2.63±0.26a | 1.61±0.03b | 3.38±0.77a | 3.64±1.39a |
总挥发性脂肪酸Total volatile fatty acids (TVFA, mmol·L-1) | 44.12±4.73a | 40.13±1.60b | 36.73±5.33a | 36.68±4.17a | 36.66±3.23a | 33.37±4.49a |
乙酸Acetic acid (%) | 70.60±10.49a | 73.91±2.85a | 72.85±3.37a | 73.48±0.54a | 78.87±1.41a | 78.95±2.68a |
丙酸Propionic acid (%) | 18.14±6.87a | 16.46±1.82a | 17.49±2.55a | 16.21±0.66a | 14.72±0.87a | 14.87±2.03a |
异丁酸Isobutyric acid (%) | 0.91±0.56a | 0.50±0.13a | 0.53±0.18a | 0.75±0.11a | 0.51±0.17a | 0.70±0.16a |
丁酸Butyric acid (%) | 7.91±2.56a | 7.15±1.83a | 6.10±0.70b | 7.37±0.45a | 2.79±1.10a | 3.16±0.83a |
异戊酸Isovaleric acid (%) | 1.26±0.56a | 1.52±0.09a | 1.20±0.35a | 1.45±0.15a | 1.11±0.50a | 1.60±0.03a |
戊酸Valeric acid (%) | 1.17±0.94a | 0.83±0.21a | 1.83±2.48a | 0.75±0.21a | 2.00±2.79a | 0.72±0.03a |
项目 Item | 瘤胃Rumen | 网胃Reticulum | 瓣胃Omasum | |||
---|---|---|---|---|---|---|
SS | CS | SS | CS | SS | CS | |
短普雷沃氏菌P. brevis | 0.25±0.05a | 0.02±0.00b | 0.04±0.01a | 0.05±0.02a | 0.05±0.04b | 0.23±0.02a |
溶纤维丁酸弧菌B. fibrisolvens | 0.13±0.02a | 0.14±0.08a | 0.29±0.08a | 0.01±0.00b | 0.14±0.04a | 0.30±0.21a |
栖普雷沃氏菌P. ruminicola | 0.62±0.10a | 0.12±0.08b | 0.15±0.07a | 0.03±0.01b | 0.10±0.05a | 0.07±0.00a |
产琥珀酸丝状杆菌F. succinogenes | 0.05±0.01a | 0.01±0.01b | 0.05±0.00a | 0.02±0.01b | 0.05±0.01a | 0.02±0.01b |
白色瘤胃球菌R.albus | 0.09±0.05a | 0.04±0.02a | 0.10±0.03a | 0.06±0.01a | 0.26±0.10a | 0.14±0.05a |
黄色瘤胃球菌R. flavefaciens | 0.17±0.05a | 0.01±0.00b | 0.45±0.06a | 0.01±0.00b | 0.09±0.03b | 0.25±0.05a |
Table 4 Effects of sweet sorghum and whole-plant corn silage on microbial expression of forestomach in mutton sheep (%)
项目 Item | 瘤胃Rumen | 网胃Reticulum | 瓣胃Omasum | |||
---|---|---|---|---|---|---|
SS | CS | SS | CS | SS | CS | |
短普雷沃氏菌P. brevis | 0.25±0.05a | 0.02±0.00b | 0.04±0.01a | 0.05±0.02a | 0.05±0.04b | 0.23±0.02a |
溶纤维丁酸弧菌B. fibrisolvens | 0.13±0.02a | 0.14±0.08a | 0.29±0.08a | 0.01±0.00b | 0.14±0.04a | 0.30±0.21a |
栖普雷沃氏菌P. ruminicola | 0.62±0.10a | 0.12±0.08b | 0.15±0.07a | 0.03±0.01b | 0.10±0.05a | 0.07±0.00a |
产琥珀酸丝状杆菌F. succinogenes | 0.05±0.01a | 0.01±0.01b | 0.05±0.00a | 0.02±0.01b | 0.05±0.01a | 0.02±0.01b |
白色瘤胃球菌R.albus | 0.09±0.05a | 0.04±0.02a | 0.10±0.03a | 0.06±0.01a | 0.26±0.10a | 0.14±0.05a |
黄色瘤胃球菌R. flavefaciens | 0.17±0.05a | 0.01±0.00b | 0.45±0.06a | 0.01±0.00b | 0.09±0.03b | 0.25±0.05a |
组别 Group | 瘤胃Rumen | 网胃Reticulum | 瓣胃Omasum |
---|---|---|---|
SS | 46.65±0.42a | 44.42±1.04a | 53.40±4.25a |
CS | 40.23±4.49b | 37.98±0.60b | 41.66±3.28b |
Table 5 Effects of sweet sorghum and whole-plant corn silage on the color of forestomach epithelium in mutton sheep (亮度L*)
组别 Group | 瘤胃Rumen | 网胃Reticulum | 瓣胃Omasum |
---|---|---|---|
SS | 46.65±0.42a | 44.42±1.04a | 53.40±4.25a |
CS | 40.23±4.49b | 37.98±0.60b | 41.66±3.28b |
前胃部位Forestomach site | 项目Item | SS | CS |
---|---|---|---|
瘤胃Rumen | 乳头长度 Length of nipples | 1631.10±1024.99a | 1622.20±1076.27a |
乳头宽度 Width of nipples | 308.70±115.88a | 249.20±122.61b | |
黏膜上皮厚度 Thickness of mucous epithelium | 113.00±9.90a | 83.14±6.25a | |
肌层厚度 Thickness of muscle layer | 913.64±169.20a | 903.80±593.58a | |
黏膜下层厚度 Thickness of submucosa | 258.99±35.00a | 255.60±63.42a | |
网胃Reticulum | 黏膜厚度 Thickness of mucous | 38.30±4.81a | 42.82±3.83a |
黏膜下层厚度 Thickness of submucosa | 133.60±23.51a | 176.94±65.09a | |
肌层厚度 Thickness of muscle layer | 888.17±116.30a | 1034.39±350.38a | |
瓣胃Omasum | 黏膜上皮厚度 Thickness of mucous epithelium | 18.27±0.10b | 20.39±1.45a |
中央肌层厚度 Thickness of central muscle | 55.07±10.91a | 61.57±10.06a | |
瓣叶肌层厚度 Thickness of omasum lamina muscle | 44.15±8.72a | 55.85±11.39a |
Table 6 Effects of sweet sorghum and whole-plant corn silage on the tissue structure of forestomach in mutton sheep (μm)
前胃部位Forestomach site | 项目Item | SS | CS |
---|---|---|---|
瘤胃Rumen | 乳头长度 Length of nipples | 1631.10±1024.99a | 1622.20±1076.27a |
乳头宽度 Width of nipples | 308.70±115.88a | 249.20±122.61b | |
黏膜上皮厚度 Thickness of mucous epithelium | 113.00±9.90a | 83.14±6.25a | |
肌层厚度 Thickness of muscle layer | 913.64±169.20a | 903.80±593.58a | |
黏膜下层厚度 Thickness of submucosa | 258.99±35.00a | 255.60±63.42a | |
网胃Reticulum | 黏膜厚度 Thickness of mucous | 38.30±4.81a | 42.82±3.83a |
黏膜下层厚度 Thickness of submucosa | 133.60±23.51a | 176.94±65.09a | |
肌层厚度 Thickness of muscle layer | 888.17±116.30a | 1034.39±350.38a | |
瓣胃Omasum | 黏膜上皮厚度 Thickness of mucous epithelium | 18.27±0.10b | 20.39±1.45a |
中央肌层厚度 Thickness of central muscle | 55.07±10.91a | 61.57±10.06a | |
瓣叶肌层厚度 Thickness of omasum lamina muscle | 44.15±8.72a | 55.85±11.39a |
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