Acta Prataculturae Sinica ›› 2022, Vol. 31 ›› Issue (10): 28-40.DOI: 10.11686/cyxb2021403
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Chun-wen WANG(), Fang ZHAO, Chen ZHANG, Li-na XIE(), Cheng-cang MA()
Received:
2021-11-09
Revised:
2021-12-13
Online:
2022-10-20
Published:
2022-09-14
Contact:
Li-na XIE,Cheng-cang MA
Chun-wen WANG, Fang ZHAO, Chen ZHANG, Li-na XIE, Cheng-cang MA. Effects of Caragana microphylla shrubs on the soil nitrogen-fixing microbial communities in steppe land[J]. Acta Prataculturae Sinica, 2022, 31(10): 28-40.
Fig.1 Effects of C. microphylla shrubs on species richness and abundance for soil nitrogen-fixing microbial communities in desert steppe and typical steppe (RII)
项目 Item | 土层 Soil depth | 荒漠草原Desert steppe | 典型草原Typical steppe | ||
---|---|---|---|---|---|
灌丛内Inside-shrub | 灌丛外Outside-shrub | 灌丛内Inside-shrub | 灌丛外Outside-shrub | ||
共生 Symbiotic | 0~10 cm | 链霉菌属Streptomyces 23.84 节杆菌属Arthrobacter 16.25 金黄杆菌属Chryseobacterium 14.15 短小杆菌属Curtobacterium 10.58 | 芽孢杆菌属Bacillus 46.89 链霉菌属Streptomyces 25.73 | 链霉菌属Streptomyces 36.88 微杆菌属Microbacterium 21.25 芽孢杆菌属Bacillus 11.80 | 链霉菌属Streptomyces 47.81 微杆菌属Microbacterium 15.07 芽孢杆菌属Bacillus 14.88 |
10~20 cm | 链霉菌属Streptomyces 33.70 节杆菌属Arthrobacter 16.08 | 芽孢杆菌属Bacillus 44.59 链霉菌属Streptomyces 30.46 类芽孢杆菌属Paenibacillus 11.17 | 链霉菌属Streptomyces 34.90 微杆菌属Microbacterium 20.21 芽孢杆菌属Bacillus 15.30 节杆菌属Arthrobacter 14.83 | 链霉菌属Streptomyces 33.67 芽孢杆菌属Bacillus 23.68 微杆菌属Microbacterium 15.02 节杆菌属Arthrobacter 14.09 | |
30~40 cm | 链霉菌属Streptomyces 30.69 假单胞菌属Pseudomonas 13.43 金黄杆菌属Chryseobacterium 12.32 | 芽孢杆菌属Bacillus 52.48 链霉菌属Streptomyces 28.36 | 链霉菌属Streptomyces 37.46 节杆菌属Arthrobacter 36.84 芽孢杆菌属Bacillus 19.43 | 链霉菌属Streptomyces 42.52 芽孢杆菌属Bacillus 21.29 微杆菌属Microbacterium 20.95 | |
自生 Free-living | 0~10 cm | 芽孢杆菌属Bacillus 49.26 链霉菌属Streptomyces 23.10 中华根瘤菌属Sinorhizobium 15.67 | 链霉菌属Streptomyces 85.37 | 链霉菌属Streptomyces 49.87 中华根瘤菌属Sinorhizobium 21.97 | 链霉菌属Streptomyces 83.02 |
10~20 cm | 芽孢杆菌属Bacillus 49.98 链霉菌属Streptomyces 29.93 | 链霉菌属Streptomyces 89.78 | 链霉菌属Streptomyces 54.26 中华根瘤菌属Sinorhizobium 28.67 | 链霉菌属Streptomyces 80.95 中华根瘤菌属Sinorhizobium 10.44 | |
30~40 cm | 链霉菌属Streptomyces 48.36 芽孢杆菌属Bacillus 41.38 | 链霉菌属Streptomyces 93.81 | 链霉菌属Streptomyces 41.11 芽孢杆菌属Bacillus 36.40 中华根瘤菌属Sinorhizobium 12.74 | 链霉菌属Streptomyces 86.31 |
Table 1 Dominant genus and relative abundance of soil nitrogen-fixing microbial communities outside and inside C. microphylla shrub canopies in desert steppe and typical steppe (%)
项目 Item | 土层 Soil depth | 荒漠草原Desert steppe | 典型草原Typical steppe | ||
---|---|---|---|---|---|
灌丛内Inside-shrub | 灌丛外Outside-shrub | 灌丛内Inside-shrub | 灌丛外Outside-shrub | ||
共生 Symbiotic | 0~10 cm | 链霉菌属Streptomyces 23.84 节杆菌属Arthrobacter 16.25 金黄杆菌属Chryseobacterium 14.15 短小杆菌属Curtobacterium 10.58 | 芽孢杆菌属Bacillus 46.89 链霉菌属Streptomyces 25.73 | 链霉菌属Streptomyces 36.88 微杆菌属Microbacterium 21.25 芽孢杆菌属Bacillus 11.80 | 链霉菌属Streptomyces 47.81 微杆菌属Microbacterium 15.07 芽孢杆菌属Bacillus 14.88 |
10~20 cm | 链霉菌属Streptomyces 33.70 节杆菌属Arthrobacter 16.08 | 芽孢杆菌属Bacillus 44.59 链霉菌属Streptomyces 30.46 类芽孢杆菌属Paenibacillus 11.17 | 链霉菌属Streptomyces 34.90 微杆菌属Microbacterium 20.21 芽孢杆菌属Bacillus 15.30 节杆菌属Arthrobacter 14.83 | 链霉菌属Streptomyces 33.67 芽孢杆菌属Bacillus 23.68 微杆菌属Microbacterium 15.02 节杆菌属Arthrobacter 14.09 | |
30~40 cm | 链霉菌属Streptomyces 30.69 假单胞菌属Pseudomonas 13.43 金黄杆菌属Chryseobacterium 12.32 | 芽孢杆菌属Bacillus 52.48 链霉菌属Streptomyces 28.36 | 链霉菌属Streptomyces 37.46 节杆菌属Arthrobacter 36.84 芽孢杆菌属Bacillus 19.43 | 链霉菌属Streptomyces 42.52 芽孢杆菌属Bacillus 21.29 微杆菌属Microbacterium 20.95 | |
自生 Free-living | 0~10 cm | 芽孢杆菌属Bacillus 49.26 链霉菌属Streptomyces 23.10 中华根瘤菌属Sinorhizobium 15.67 | 链霉菌属Streptomyces 85.37 | 链霉菌属Streptomyces 49.87 中华根瘤菌属Sinorhizobium 21.97 | 链霉菌属Streptomyces 83.02 |
10~20 cm | 芽孢杆菌属Bacillus 49.98 链霉菌属Streptomyces 29.93 | 链霉菌属Streptomyces 89.78 | 链霉菌属Streptomyces 54.26 中华根瘤菌属Sinorhizobium 28.67 | 链霉菌属Streptomyces 80.95 中华根瘤菌属Sinorhizobium 10.44 | |
30~40 cm | 链霉菌属Streptomyces 48.36 芽孢杆菌属Bacillus 41.38 | 链霉菌属Streptomyces 93.81 | 链霉菌属Streptomyces 41.11 芽孢杆菌属Bacillus 36.40 中华根瘤菌属Sinorhizobium 12.74 | 链霉菌属Streptomyces 86.31 |
Fig.3 Non-metric multi-dimensional scale analysis (NMDS) and ANOSIM test of the soil nitrogen-fixing microbial community composition outside and inside C. microphylla shrub canopies in desert steppe and typical steppe
Fig.4 The Jaccard dissimilarity index of soil nitrogen-fixing microbial communities between outside and inside C. microphylla shrub canopies in desert steppe and typical steppe
Fig.5 The Jaccard dissimilarity index of nitrogen-fixing microbial communities between desert steppe and typical steppe outside or inside C. microphylla shrub canopies
项目 Item | 土层 Soil depth | 荒漠草原Desert steppe | 典型草原Typical steppe | ||||
---|---|---|---|---|---|---|---|
正选择 Positive-selective | 负选择 Negative-selective | 总选择 Total selective | 正选择 Positive-selective | 负选择 Negative-selective | 总选择 Total selective | ||
共生Symbiotic | 0~10 cm | 52.94 | 17.65 | 70.59 | 25.00 | 12.50 | 37.50 |
10~20 cm | 27.78 | 16.67 | 44.45 | 6.67 | 20.00 | 26.67 | |
30~40 cm | 20.00 | 20.00 | 40.00 | 14.29 | 28.57 | 42.86 | |
自生Free-living | 0~10 cm | 54.55 | 0.00 | 54.55 | 55.56 | 0.00 | 55.56 |
10~20 cm | 44.44 | 0.00 | 44.44 | 37.50 | 12.50 | 50.00 | |
30~40 cm | 33.33 | 16.67 | 50.00 | 60.00 | 20.00 | 80.00 |
Table 2 The percentage of selective species of C. microphylla shrub canopies on soil nitrogen-fixing microbial communities in desert steppe and typical steppe (%)
项目 Item | 土层 Soil depth | 荒漠草原Desert steppe | 典型草原Typical steppe | ||||
---|---|---|---|---|---|---|---|
正选择 Positive-selective | 负选择 Negative-selective | 总选择 Total selective | 正选择 Positive-selective | 负选择 Negative-selective | 总选择 Total selective | ||
共生Symbiotic | 0~10 cm | 52.94 | 17.65 | 70.59 | 25.00 | 12.50 | 37.50 |
10~20 cm | 27.78 | 16.67 | 44.45 | 6.67 | 20.00 | 26.67 | |
30~40 cm | 20.00 | 20.00 | 40.00 | 14.29 | 28.57 | 42.86 | |
自生Free-living | 0~10 cm | 54.55 | 0.00 | 54.55 | 55.56 | 0.00 | 55.56 |
10~20 cm | 44.44 | 0.00 | 44.44 | 37.50 | 12.50 | 50.00 | |
30~40 cm | 33.33 | 16.67 | 50.00 | 60.00 | 20.00 | 80.00 |
项目 Item | 土层 Soil depth | 灌丛 Shrub canopies | 气候干旱 Climate aridity |
---|---|---|---|
共生 Symbiotic | 0~10 cm | 45.27 | 16.67 |
10~20 cm | 53.61 | 26.47 | |
30~40 cm | 69.03 | 0.00 | |
自生 Free-living | 0~10 cm | 74.47 | 28.69 |
10~20 cm | 33.61 | 7.00 | |
30~40 cm | 49.39 | 0.00 |
Table 3 The relative effects of shrub canopies and climatic aridity on soil nitrogen-fixing microbial communities (%)
项目 Item | 土层 Soil depth | 灌丛 Shrub canopies | 气候干旱 Climate aridity |
---|---|---|---|
共生 Symbiotic | 0~10 cm | 45.27 | 16.67 |
10~20 cm | 53.61 | 26.47 | |
30~40 cm | 69.03 | 0.00 | |
自生 Free-living | 0~10 cm | 74.47 | 28.69 |
10~20 cm | 33.61 | 7.00 | |
30~40 cm | 49.39 | 0.00 |
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