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Acta Prataculturae Sinica ›› 2009, Vol. 18 ›› Issue (2): 212-215.

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A study on callus induction and redifferentiation of clover

WANG You-sheng1, WANG Ying2, LI Yang-chun3   

  1. 1.Dingxi Agricutural Technology Extensional Station, Dingxi 743000, China;
    2.Dongguan Botanical
    Garden, Dongguan 523000, China;
    3.Gansu Agricultural University, Lanzhou 730070, China
  • Received:2008-06-20 Online:2009-04-20 Published:2009-04-20

Abstract: The hypocotyls, leaf and cotyledon of four varieties of clover (Trifolum) were used to study the effect of hormone concentrations on callus induction and redifferentiation. Different varieties and explants each had different induction requirements. The hypocotyl callus induction rate of Trifolium hybridum was high (96.7%), while that of somatic embryogenesis was 33.4%. The best medium for embryogenic callus induction was modified SH+2,4-D 4.0 mg/L+KT 1 mg/L, while for embryoid redifferentiation and plant regeneration it was MSO.

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