Abstract: Plate confrontation test of 7 isolates of fungal endophytes on Brachiaria spp. against pathogenic fungi Drechslera sp. and Rhizoctonia solani on PDA showed that most of the isolates had inhibition effect to the both pathogenic fungi. Different isolates had different antagonistic effect to the same pathogenic fungi, and the same isolate also had different antagonistic effect to different pathogenic fungi. Among of endophyte isolates, EB6780.501 and EH32a showed the best inhibition effect to both D. sp. and R. solani. In vivo test showed endophyte infected plants clearly resistance to foliar blight caused by R. solani. But the effectiveness became less as time going on after inoculation of R. solani. There was correlation between in vitro and in vivo test, the isolates with strong in vitro inhibitory effects also showed strong plant protection. Since in vitro inhibition test is much easier and simpler to do than in vivo test, it is better to make an inhibition in vitro test before in vivo in the selection of endophyte strains with disease-resistance.