Protoplast culture and plant regeneration of Zygophyllum xanthoxylum

Abstract

Abstract: An efficient protocol for plant regeneration from protoplasts of Zygophyllum xanthoxylum was established. The friable calli induced from segments of cotyledon were used for protoplast isolation through enzyme digestion. The effects of different media and plating densities on protoplast divisions and plant regeneration were studied. Sustained cell divisions and colony formation from the protoplasts of Z. xanthoxylum were obtained by a DPD medium containing 1.0 mg/L 2, 4-dichlorophenoxyacetic acid (2, 4-D), 0.2 mg/L 6-benzylaminopurine (6-BA), 0.2 mg/L kinetin (KT), 0.5 mol/L mannitol, 500 mg/L casein hydrolysate, and 2% (W/V) sucrose at a plating density of 2×10⁵ /mL. The division frequency was 28.4%. Regenerated plantlets were obtained when these calli were transferred to MS medium containing 1.0 mg/L 6-BA and 0.5 mg/L (NAA).

CLC Number:

WANG Ying-hua, CHEN Gang, JIA Jing-fen, HAO Jian-guo. Protoplast culture and plant regeneration of Zygophyllum xanthoxylum[J]. Acta Prataculturae Sinica, 2009, 18(3): 110-116.

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